Research on the printability of hydrogels in 3D bioprinting.
Research on the printability of hydrogels in 3D bioprinting.
复制标题
研究3D生物打印中水凝胶的可打印性。
DOI：
10.1038/srep29977
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发表时间：
2016-07-20
影响因子：
4.6
通讯作者：
Fu J
中科院分区：
文献类型：
--
作者：
He Y;Yang F;Zhao H;Gao Q;Xia B;Fu J
作为生物相容性材料，水凝胶已被广泛用于三维（3D）生物打印/器官打印以加载用于组织工程的细胞。在打印模拟器官结构期间精确控制水凝胶沉积是重要的。然而，关于印刷参数的水凝胶的印刷适性很少被提及。本文系统地研究了水凝胶的可印刷性，从印刷线条（一维，1D结构）到印刷晶格/薄膜（二维，2D结构）和印刷3D结构，特别关注精确印刷。经过一系列的实验，我们发现了一些重要的因素，如空气压力，进给速度，甚至打印距离和预期结构的打印质量之间的关系。由于水凝胶在交叉处扩散，在晶格结构印刷中观察到哑铃形状。相邻层的塌陷和融合会导致Z方向的误差积累，这是导致打印失败的重要因素。最后，我们成功地展示了一个3D打印水凝胶支架，通过协调与所有参数。打印后的细胞活力与铸造进行了比较，结果表明，我们的生物打印方法几乎没有对细胞造成额外的损害。
As the biocompatible materials, hydrogels have been widely used in three- dimensional (3D) bioprinting/organ printing to load cell for tissue engineering. It is important to precisely control hydrogels deposition during printing the mimic organ structures. However, the printability of hydrogels about printing parameters is seldom addressed. In this paper, we systemically investigated the printability of hydrogels from printing lines (one dimensional, 1D structures) to printing lattices/films (two dimensional, 2D structures) and printing 3D structures with a special attention to the accurate printing. After a series of experiments, we discovered the relationships between the important factors such as air pressure, feedrate, or even printing distance and the printing quality of the expected structures. Dumbbell shape was observed in the lattice structures printing due to the hydrogel diffuses at the intersection. Collapses and fusion of adjacent layer would result in the error accumulation at Z direction which was an important fact that could cause printing failure. Finally, we successfully demonstrated a 3D printing hydrogel scaffold through harmonize with all the parameters. The cell viability after printing was compared with the casting and the results showed that our bioprinting method almost had no extra damage to the cells.