The dynamic change of antibody index against Covid-19 is a powerful diagnostic tool for the early phase of the infection and salvage PCR assay errors.
The dynamic change of antibody index against Covid-19 is a powerful diagnostic tool for the early phase of the infection and salvage PCR assay errors.
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DOI：
10.1016/j.jmii.2020.12.009
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发表时间：
2021-10
期刊：
影响因子：
--
通讯作者：
Miyashita Y
中科院分区：
文献类型：
--
作者：
Omata M;Hirotsu Y;Sugiura H;Maejima M;Nagakubo Y;Amemiya K;Hayakawa M;Tsutsui T;Kakizaki Y;Mochizuki H;Miyashita Y
目前，PCR检测是诊断新冠肺炎的金标准。然而，它需要鼻咽拭子，昂贵的仪器和专业知识。甚至会导致PCR错误。我们在36名随访患者和1879名对照（医务人员）中验证了抗体测定（Roche）。在1879名医务人员中，只有2名（0.11%）被截断指数（COI; 1.0）（均值± SD，0.094 ± 0.047）检测为阳性。36例患者分为三组：A组，4例来自钻石公主号游轮，B组，2例在非洲感染，C组，30例在日本感染。住院前在外部实验室进行PCR检测，住院后在室内重复检测。在36例患者中，4/4来自船上，0/2来自非洲，5/30来自日本。来自非洲的两个显示COI增加，并在第8天和第13天变为阳性。30名日本人被分为两组，例如，23在病毒复制活跃的情况下，COI在3天内动态增加至84.4（C组）。在其余7例（7/30，23%）（C '组）中，内部PCR检测未出现抗体升高或阳性，表明开始时PCR的假阳性结果。这种抗体检测具有广泛的动态范围的COI，因此，可以在感染早期使用。这也可以补充，甚至有助于避免可能的PCR错误。因此，这可以作为一个强大的诊断工具，需要在诊所和医院的第一线。
Currently, PCR assay is a golden standard for diagnosis of Covid-19. However, it needs nasopharyngeal swabs, expensive instruments and expertise. It even causes PCR errors. We validated the antibody assay (Roche) in 36 followed patients and 1879 controls (medical staffs). Of 1879 medical staffs, only two (0.11%) were positive by Cut off Index (COI; 1.0) (mean ± SD, 0.094 ± 0.047). Thirty six patients were composed of three groups; Group A,4 from Diamond Princess cruise ship, Group B, 2 infected in Africa, and Group C, 30 infected in Japan. PCR assays were conducted at outside laboratories before and repeated in house after hospitalized. Of 36 at admission, positive antibody was seen in 4/4 from the ship, 0/2 from Africa, and 5/30 from Japan. Two from Africa showed the increase of COI and became positive on days 8 and 13. Thirty Japanese was divided in two groups, e.g., 23 showed dynamic increase of COI up to 84.4 within 3 days while active virus replication present (Group C). In remaining 7 (7/30, 23%) (Group C'), no rise of antibody nor positive in house PCR assays, indicative of false positive results of PCR at the beginning. This antibody testing has a wide dynamic ranges of COI and, thus, could be utilized in the early infection phase. This may also compliment and even help to avoid possible PCR errors. Therefore, this can serve as a powerful diagnostic tool, needed in the frontline of the clinic and hospitals.
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影响因子：
168.9
作者：
Huang, Chaolin;Wang, Yeming;Cao, Bin
通讯作者：
Cao, Bin
影响因子：
3.1
作者：
Hirotsu, Yosuke;Mochizuki, Hitoshi;Omata, Masao
通讯作者：
Omata, Masao
DOI：
10.1016/s0140-6736(20)32009-2
发表时间：
2020-10-24
期刊：
Lancet (London, England)
影响因子：
--
作者：
Anand S;Montez-Rath M;Han J;Bozeman J;Kerschmann R;Beyer P;Parsonnet J;Chertow GM
通讯作者：
Chertow GM
影响因子：
2.2
作者：
Yu, H
通讯作者：
Yu, H
影响因子：
168.9
作者：
Pollan, Marina;Perez-Gomez, Beatriz;Yotti, Raquel
通讯作者：
Yotti, Raquel