Identification of antigens and antibodies specific for Pneumocystis carinii.
Identification of antigens and antibodies specific for Pneumocystis carinii.
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卡氏肺孢子虫特异性抗原和抗体的鉴定。
DOI：
10.4049/jimmunol.140.6.2023
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发表时间：
1988
影响因子：
4.4
通讯作者：
Henry Masur
中科院分区：
文献类型：
--
作者：
J. Kovacs;J. Halpern;J. Swan;Joel Moss;Joseph E. Parrillo;Henry Masur
为加深对卡氏肺孢子虫及其与宿主相互作用的了解，采用免疫印迹方法，在卡氏肺孢子虫生物提取物PAGE后，对鼠类和人卡氏肺孢子虫进行了抗原鉴定。M.W.卡氏肺吸虫和人卡氏肺吸虫的主要抗原分别为45,000,110,000和49,000至64,000条宽带和22,000，24,000和35,000至45,000道尔顿的宽带。人和大鼠的肺孢子虫在抗原性上并不相同。免疫印迹法检测79只大鼠中有18只检出卡氏肺孢子虫特异性抗体。33份成人血清中有32份检测到抗卡氏肺孢子虫抗原的特异性抗体，而8份婴幼儿血清中仅有1份检测到特异性抗体。在14例无卡氏肺孢子虫肺炎病史的成人和所有19例新近确诊的卡氏肺孢子虫肺炎患者中的13例血清中发现了特异性抗体，其中包括9例与艾滋病相关的卡氏肺孢子虫肺炎患者。这项研究的结果支持了先前关于很大比例的成虫接触过卡氏肺吸虫的说法，并为进一步研究宿主-卡氏肺吸虫提供了基础。隆突相互作用。
To increase understanding of Pneumocystis carinii and its interaction with its hosts, Ag specific for rodent and human P. carinii were identified by the immunoblot method after PAGE of P. carinii organism extracts. The m.w. of the major Ag of rat P. carinii were 45,000, 110,000, and a broad band of 49,000 to 64,000, and of human P. carinii were 22,000, 24,000, and a broad band of 35,000 to 45,000 daltons. Human and rat pneumocystis were not antigenically identical. Specific antibodies against rat P. carinii Ag were found in 18 of 79 rats by the immunoblot method. Specific antibodies against human P. carinii Ag were found in 32 of 33 adult human sera, but in only 1 of 8 sera from infants and children. Specific antibodies were found in sera of 13 of the 14 adults with no history of P. carinii pneumonia, and all 19 patients with recently diagnosed P. carinii pneumonia, including 9 patients with P. carinii pneumonia associated with AIDS. The results of this study support previous suggestions that a large proportion of adults have been exposed to P. carinii and provide a basis for the further investigations of host-P. carinii interactions.