MinION Analysis and Reference Consortium: Phase 1 data release and analysis.
MinION Analysis and Reference Consortium: Phase 1 data release and analysis.
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DOI：
10.12688/f1000research.7201.1
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
MinION Analysis and Reference Consortium
中科院分区：
文献类型：
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作者：
Ip CLC;Loose M;Tyson JR;de Cesare M;Brown BL;Jain M;Leggett RM;Eccles DA;Zalunin V;Urban JM;Piazza P;Bowden RJ;Paten B;Mwaigwisya S;Batty EM;Simpson JT;Snutch TP;Birney E;Buck D;Goodwin S;Jansen HJ;O'Grady J;Olsen HE;MinION Analysis and Reference Consortium
一种具有高通量情境测序能力的小型化DNA测序设备的出现，代表了下一代大规模测序工具。2014年4月，牛津纳米孔技术公司启动了MinION™准入计划（MAP），使公众能够使用他们的USB接口小型测序设备。MinION分析与参考联盟（MARC）由MAP的一部分参与者组成，其目的是评估并向社区提供标准操作规程和参考数据。本研究被设想为一个多阶段项目，它向全球社区提供了来自MARC的第一阶段数据，其中在多个地点对MinION性能的可重复性进行了评估。两大洲的五个实验室使用大肠杆菌K - 12的对照菌株生成数据，按照修订后的牛津纳米孔技术公司（ONT）操作规程制备样本并进行测序。在此，我们提供了所使用操作规程的详细信息，以及对典型运行特征的初步分析，包括所产生数据的一致性、速率、量和质量。对本文所呈现的第一阶段数据的进一步分析，以及MARC在大肠杆菌第二阶段的额外实验已经在进行中，以确定改进和提高MinION性能的方法。
The advent of a miniaturized DNA sequencing device with a high-throughput contextual sequencing capability embodies the next generation of large scale sequencing tools. The MinION™ Access Programme (MAP) was initiated by Oxford Nanopore Technologies™ in April 2014, giving public access to their USB-attached miniature sequencing device. The MinION Analysis and Reference Consortium (MARC) was formed by a subset of MAP participants, with the aim of evaluating and providing standard protocols and reference data to the community. Envisaged as a multi-phased project, this study provides the global community with the Phase 1 data from MARC, where the reproducibility of the performance of the MinION was evaluated at multiple sites. Five laboratories on two continents generated data using a control strain of Escherichia coli K-12, preparing and sequencing samples according to a revised ONT protocol. Here, we provide the details of the protocol used, along with a preliminary analysis of the characteristics of typical runs including the consistency, rate, volume and quality of data produced. Further analysis of the Phase 1 data presented here, and additional experiments in Phase 2 of E. coli from MARC are already underway to identify ways to improve and enhance MinION performance.