Zeptomole Detection of an Enzyme by a Simple Colorimetric Method
Zeptomole Detection of an Enzyme by a Simple Colorimetric Method
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DOI：
10.2116/analsci.21n009
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发表时间：
2021-10-01
影响因子：
1.6
通讯作者：
Ito, Etsuro
中科院分区：
文献类型：
--
作者：
Iha, Kanako;Kyosei, Yuta;Ito, Etsuro
酶免疫分析是将酶（例如碱性磷酸酶，ALP）与抗体结合，是一种精确而简单的蛋白质检测方法。低浓度酶的精确测量可以实现超灵敏的蛋白质检测。将磷酸化底物应用于 ALP，然后在硫代烟酰胺-腺嘌呤二核苷酸循环中使用去磷酸化底物，可对 ALP 进行简单而精确的定量。我们描述了一种使用简单的比色法在 zeptomole 水平检测 ALP 的协议。
An enzyme immunoassay, in which an enzyme (e.g., alkaline phosphatase, ALP) is conjugated with an antibody, is a precise and simple protein detection method. Precise measurements of enzymes at low concentrations allow for ultrasensitive protein detection. The application of a phosphorylated substrate to ALP, followed by using a dephosphorylated substrate in thionicotinamide-adenine dinucleotide cycling, provides a simple and precise quantification of ALP. We describe a protocol for detecting ALP at the zeptomole level using a simple colorimetric method.