Perturbations in Blood Phosphatidylcholine and Sphingomyelin Fatty Acid Composition in a Sample Population of Cigarette Smokers
Perturbations in Blood Phosphatidylcholine and Sphingomyelin Fatty Acid Composition in a Sample Population of Cigarette Smokers
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DOI：
10.1007/s12291-013-0327-y
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发表时间：
2013-10-01
影响因子：
2.1
通讯作者：
Bueno, Allain A.
中科院分区：
文献类型：
--
作者：
Ionescu, Nicoleta;de Freitas, Caterina;Bueno, Allain A.
二十二碳六烯酸（DHA）和花生四烯酸（AA）是多不饱和脂肪酸（PUFAs），是脑组织和神经系统的主要成分，也是许多具有抗炎、神经保护等作用的生物活性代谢物的前体。由于多不饱和脂肪酸对过氧化反应高度敏感，我们假设吸烟者是否会改变血浆和红细胞磷脂中的多不饱和脂肪酸水平。招募了来自印度、斯里兰卡或孟加拉国遗传背景的成年男性，他们报告每周吸烟20至60支。对照组由匹配的非吸烟者组成。采集血样，提取血浆和红细胞总脂质，用薄层色谱法分离磷脂，用气相色谱法分析脂肪酸含量。在吸烟者中，血浆和红细胞磷脂酰胆碱中的AA前体二高-γ-亚麻酸显著减少。红细胞鞘磷脂中AA和DHA显著减少。在相对年轻的人群中，相对短期的吸烟影响了血浆和红细胞磷脂的脂肪酸组成，这些磷脂在神经组织组成、细胞信号传导、细胞生长、细胞内运输、神经保护和炎症中起作用。由于脂质过氧化在动脉粥样硬化和阿尔茨海默病等神经退行性疾病的发病机制中起关键作用，因此吸烟的早期影响可能与此类疾病的发展有关。
Docosahexaenoic (DHA) and arachidonic acids (AA) are polyunsaturated fatty acids (PUFAs), major components of brain tissue and neural systems, and the precursors of a number of biologically active metabolites with functions in inflammation resolution, neuroprotection and other actions. As PUFAs are highly susceptible to peroxidation, we hypothesised whether cigarette smokers would present altered PUFAs levels in plasma and erythrocyte phospholipids. Adult males from Indian, Sri-Lankan or Bangladeshi genetic backgrounds who reported smoking between 20 and 60 cigarettes per week were recruited. The control group consisted of matched non-smokers. A blood sample was taken, plasma and erythrocyte total lipids were extracted, phospholipids were separated by thin layer chromatography, and the fatty acid content analysed by gas chromatography. In smokers, dihomo-gamma-linolenic acid, the AA precursor, was significantly reduced in plasma and erythrocyte phosphatidylcholine. AA and DHA were significantly reduced in erythrocyte sphingomyelin. Relatively short term smoking has affected the fatty acid composition of plasma and erythrocyte phospholipids with functions in neural tissue composition, cell signalling, cell growth, intracellular trafficking, neuroprotection and inflammation, in a relatively young population. As lipid peroxidation is pivotal in the pathogenesis of atherosclerosis and neurodegenerative diseases such as Alzheimer disease, early effects of smoking may be relevant for the development of such conditions.