Genomic organization and localization of mouse Nma/BAMBI: Possible implications related to ameloblastoma formation
Genomic organization and localization of mouse Nma/BAMBI: Possible implications related to ameloblastoma formation
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DOI：
10.1080/03008200290000600
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发表时间：
2002-04-01
影响因子：
2.9
通讯作者：
MacDougall, M
中科院分区：
文献类型：
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作者：
Knight, C;Papagerakis, P;MacDougall, M
我们的实验室已经确定了一个小鼠cDNA克隆的DNA序列和转录表达模式，称为Nma/BAMBI。该克隆编码一种高度保守的蛋白质，在预测的氨基酸水平上与人同源物（称为Nma）具有89%的同一性，与非洲爪蟾同源物（称为BAMBI）具有78%的相似性。Nma/BAMBI编码260个氨基酸的跨膜蛋白，其与转化生长因子（TGF）β I型受体家族具有同源性。这种蛋白质含有一个胞外配体结合结构域，一个24个氨基酸的跨膜结构域，和一个短的胞内结构域，缺乏一个功能性丝氨酸/苏氨酸激酶结构域。据信，Nma/BAMBI在发育期间TGF β信号转导途径的负调节中是重要的，并且在肿瘤进展中具有意义。我们已经确定了小鼠Nma/BAMBI基因的基因组结构，并确认了人类10号染色体和小鼠2号染色体的染色体定位。此外，我们报告的生产和利用的抗肽抗体的成釉细胞瘤的初步免疫组织化学分析。
Our laboratory has determined the DNA sequence and transcriptional expression pattern of a mouse cDNA clone termed Nma/BAMBI. This clone encodes a highly conserved protein with 89% identity to the human homologue (termed Nma) and 78% similarity to the Xenopus homologue (termed BAMBI) at the predicted amino acid level. Nma/BAMBI encodes a 260-amino-acid transmembrane protein that has homology to the transforming growth factor (TGF) beta type I receptor family. This protein contains an extracellular ligand binding domain, a 24-amino-acid transmembrane domain, and a short intracellular domain that lacks a functional serine/threonine kinase domain. It is believed that Nma/BAMBI is important in the negative regulation of TGFbeta signal transduction pathways during development and has implications in tumor progression. We have determined the genomic organization of the mouse Nma/BAMBI gene and confirmed the chromosomal mapping to human chromosome 10 and mouse chromosome 2. Furthermore, we report the production and utilization of an anti-peptide antibody in preliminary immunohistochemical analysis of an ameloblastoma.