A viral disease occurring in cultured carp Cyprinus carpio in Korea
A viral disease occurring in cultured carp Cyprinus carpio in Korea
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DOI：
10.3147/jsfp.36.147
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发表时间：
2001-09-01
期刊：
影响因子：
0.6
通讯作者：
Chun, SK
中科院分区：
文献类型：
--
作者：
Oh, MJ;Jung, SJ;Chun, SK
1998年以来，韩国不同年龄的鲤鱼发生了一种新的高死亡率疾病。该病的临床症状包括垂死的鱼在水面游动不规律，呼吸困难，身体和鳃上有浓雾状的粘液，少数病例还发现真皮溃疡。对采自各地的鱼类样品进行了微生物学、寄生虫学和病毒学调查。虽然从一些鱼中发现了细菌和原生动物寄生虫，但它们的出现并不一致。在组织学上，肾和脾表现出明显的变化。将病鱼的组织滤液接种到不同的鱼类细胞系中，仅在FHM中显示出细胞病变效应。感染FHM细胞的超薄切片在电子显微镜下可见明显的细胞质病毒样颗粒，直径70-80 nm。浸泡感染试验，用从受感染的FHM细胞中获得的10(3.0)TCID(50)/毫升的病毒悬液，对以色列鲤鱼和普通鲤鱼的死亡率分别为100%和80%。这是韩国首例在养殖鲤鱼中报告的病毒性疾病流行性传染病。
A new disease with high mortalities has been occurring in summer scince 1998 in different ages of carp Cyprinus carpio cultured in Korea. The clinical signs of the disease included erratic swimming of moribund fish at the water surface, respiratory distress, presence of thick and foggy mucus covering the body and gills, Dermal ulcers were also noticed in a few cases. Microbiological, parasitological and virological investigations were carried out on the fish samples collected from various localities. Although bacteria and a protozoan parasite were recovered from some fish, their occurrences were not consistent. Histologically, the kidney and spleen showed discernible changes. Inoculation of tissue filtrates from affected fish to various fish cell lines showed cytopathic effects only in FHM. Ultra-thin sections of the infected FHM cells under an electron microscope revealed distinct cytoplasmic virus-like particles with a diameter of 70-80 nm. Immersion infection trials, using a viral suspension of 10(3.0)TCID(50)/mL obtained from the infected FHM cells, produced 100 and 80% mortalities in Israel carp and common carp, respectively. This is the first epizootic of viral disease reported among cultured carp in Korea.