Effect of increased polyamine biosynthesis on stress responses in transgenic tobacco by introduction of human S-adenosylmethionine gene
Effect of increased polyamine biosynthesis on stress responses in transgenic tobacco by introduction of human S-adenosylmethionine gene
复制标题
DOI：
10.1016/s0168-9452(03)00030-x
复制
发表时间：
2003-05
期刊：
影响因子：
5.2
通讯作者：
B. Waie;M. Rajam
中科院分区：
文献类型：
--
作者：
B. Waie;M. Rajam
将人S-腺苷甲硫氨酸脱羧酶（samdc; EC 4.1.1.50）基因在组成型启动子（CaMV 35 S）的控制下引入烟草（烟草变种）中。xanthi）导致多胺生物合成增加。亚精胺和腐胺滴度增加的转基因株系，特别是在共轭形式，表现出耐盐性和干旱，以及由大丽轮枝菌和尖孢镰刀菌引起的真菌枯萎病。这是第一个报告，在转基因植物的多胺生物合成基因转移后，已观察到的非生物和生物胁迫的耐受性。
Introduction of a human S-adenosylmethionine decarboxylase (samdc; EC 4.1.1.50) gene under the control of a constitutive promoter (CaMV35S) in tobacco (Nicotiana tabacum var. xanthi) led to increased polyamine biosynthesis. Transgenic lines with increasing spermidine and putrescine titres, especially in the conjugated forms, exhibited tolerance to salinity and drought as well as to fungal wilts caused by Verticillium dahliae and Fusarium oxysporum. This is the first report where tolerance to both abiotic and biotic stresses has been observed in transgenic plants following transfer of a polyamine biosynthesis gene.