Bright Lu(2) O(3) :Eu Thin-Film Scintillators for High-Resolution Radioluminescence Microscopy.
Bright Lu(2) O(3) :Eu Thin-Film Scintillators for High-Resolution Radioluminescence Microscopy.
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DOI：
10.1002/adhm.201500372
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发表时间：
2015-10
影响因子：
10
通讯作者：
Pratx G
中科院分区：
文献类型：
--
作者：
Sengupta D;Miller S;Marton Z;Chin F;Nagarkar V;Pratx G
我们研究了用于单细胞放射性核素成像的新型薄膜 Lu2O3:Eu 闪烁体的性能。实时成像异质细胞群的代谢特性是具有临床意义的重要挑战。我们开发了一种称为放射发光显微镜的创新技术，可以定量、灵敏地测量单细胞中放射性核素的吸收。该技术最重要的组成部分是闪烁体，它将放射性衰变过程中释放的能量转化为发光信号。成像系统的灵敏度和空间分辨率主要取决于闪烁体的特性，即所使用的材料及其几何结构。使用传统方法制造的闪烁体相对较厚，因此不能提供最佳的空间分辨率。我们将薄膜 Lu2O3:Eu 闪烁体与用于放射发光成像的传统 500 μm 厚 CdWO4 闪烁体进行比较。尽管它很薄，但 Lu2O3:Eu 闪烁体独特的闪烁特性使我们能够以四倍以上的灵敏度捕获单个正电子衰变，这是一项重大成就。薄膜 Lu2O3:Eu 闪烁体还可产生放射发光图像，其中单个细胞比 CdWO4 闪烁体显得更小且平均分辨率更好。与薄膜闪烁体技术相结合，放射发光显微镜可以产生有关单细胞代谢的有价值的临床相关数据。
We investigate the performance of a new thin-film Lu2O3:Eu scintillator for single-cell radionuclide imaging. Imaging the metabolic properties of heterogeneous cell populations in real time is an important challenge with clinical implications. We have developed an innovative technique called radioluminescence microscopy, to quantitatively and sensitively measure radionuclide uptake in single cells. The most important component of this technique is the scintillator, which converts the energy released during radioactive decay into luminescent signals. The sensitivity and spatial resolution of the imaging system depend critically on the characteristics of the scintillator, i.e. the material used and its geometrical configuration. Scintillators fabricated using conventional methods are relatively thick, and therefore do not provide optimal spatial resolution. We compare a thin-film Lu2O3:Eu scintillator to a conventional 500 μm thick CdWO4 scintillator for radioluminescence imaging. Despite its thinness, the unique scintillation properties of the Lu2O3:Eu scintillator allow us to capture single positron decays with over fourfold higher sensitivity, a significant achievement. The thin-film Lu2O3:Eu scintillators also yield radioluminescence images where individual cells appear smaller and better resolved on average than with the CdWO4 scintillators. Coupled with the thin-film scintillator technology, radioluminescence microscopy can yield valuable and clinically relevant data on the metabolism of single cells.