Butyrate and Propionate, Short Chain Fatty Acids, Attenuate Myocardial Damages by Inhibition of Apoptosis in a Rat Model of Ischemia-reperfusion
Butyrate and Propionate, Short Chain Fatty Acids, Attenuate Myocardial Damages by Inhibition of Apoptosis in a Rat Model of Ischemia-reperfusion
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DOI：
10.3839/jksabc.2010.088
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发表时间：
2010-10-01
影响因子：
--
通讯作者：
Lee, Jongwon
中科院分区：
文献类型：
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作者：
Lim, Sun Ha;Song, Kyung-Sik;Lee, Jongwon
谷物中的膳食纤维（DF）和抗性淀粉（RS）在大肠中发酵成短链脂肪酸（SCFA），包括丁酸酯、丙酸酯和乙酸酯，并被吸收到血液中。在大鼠缺血30 min和再灌注3 h模型中，通过在闭塞前1 h腹腔注射SCFA（10 mg/kg），评价SCFA减轻心肌损伤的有效性。丁酸盐和丙酸盐显着衰减梗死面积，心肌损伤的指标，当通过2，3，5-三苯基氯化四氮唑染色评估，减少末端脱氧核苷酸转移酶介导的dUTP缺口末端标记染色的细胞凋亡，并增加Bcl-2/Bax比值，细胞凋亡途径中的细胞存活的决定因素。此外，丁酸盐在免疫组织化学测定中显著减弱了胱天蛋白酶-3（一种刽子手胱天蛋白酶）的水平。这些结果表明，丁酸和丙酸，发酵的DF和RS在大肠中产生的，可以防止心肌梗死通过减弱细胞凋亡，可能通过抑制组蛋白脱乙酰酶。因此，通过发酵产生大量丁酸盐和丙酸盐的谷物或饮食的品种可能有利于减少心肌梗死。
Dietary fiber (DF) and resistant starch (RS) in the grains of cereals are fermented into short chain fatty acids (SCFA), including butyrate, propionate, and acetate, in the large intestine, and are absorbed into the blood. Effectiveness of SCFA in reducing myocardial damage was evaluated in a rat model of 30 min ischemia and 3 h reperfusion with administration of SCFA (10 mg/kg) by peritoneal injection 1 h before occlusion. Butyrate and propionate significantly attenuated infarct size, an indicator of myocardial damage, when assessed by 2,3,5-triphenyltetrazolium chloride staining, reduced apoptosis in terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling staining, and increased Bcl-2/Bax ratio, a determinant of cell survival in the apoptotic pathway. In addition, butyrate significantly attenuated the level of caspase-3, an executioner caspase, in an immunohistochemical assay. These results demonstrate that butyrate and propionate, produced by fermentation of DF and RS in the large intestine, could prevent myocardial infarction by attenuating apoptosis, possibly through inhibition of histone deacetylases. Thus, varieties of cereals or diets that produce a large amount of butyrate and propionate by fermentation could be beneficial in reducing myocardial infarction.