Real-time monitoring of Ralstonia solanacearum infection progress in tomato and Arabidopsis using bioluminescence imaging technology.
Real-time monitoring of Ralstonia solanacearum infection progress in tomato and Arabidopsis using bioluminescence imaging technology.
复制标题
利用生物发光成像技术实时监测番茄和拟南芥青枯菌感染进展
DOI：
10.1186/s13007-022-00841-x
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发表时间：
2022-01-15
期刊：
影响因子：
5.1
通讯作者：
Liang Y
中科院分区：
文献类型：
--
作者：
Xu C;Zhong L;Huang Z;Li C;Lian J;Zheng X;Liang Y
青枯病病原菌是青枯病的病原菌，是一种毁灭性最强的植物细菌性病原菌。近年来，利用拟南芥-R对青枯病的抗性进行了一些研究。青枯草系统。然而，青枯病菌侵染拟南芥的研究进展尚不清楚。我们通过表达基于质粒的LuxCDABE，获得了一株生物发光的青枯菌。LuxCDABE的表达不改变细菌的生长和致病性。发光菌的光强与细菌浓度在10 4~10 8cfu·m L−1范围内呈线性关系。根接种发光菌后，番茄和拟南芥中的光信号通过维管系统从根传递到茎。生物发光菌株的光强度定量准确地反映了拟南芥野生型和抗病突变体之间的抗病差异。生物发光青枯菌菌株对番茄和拟南芥中的细菌生长进行了空间和定量的检测，为今后青枯病菌与拟南芥相互作用的高通量研究提供了工具。
Ralstonia solanacearum, one of the most devastating bacterial plant pathogens, is the causal agent of bacterial wilt. Recently, several studies on resistance to bacterial wilt have been conducted using the Arabidopsis-R. solanacearum system. However, the progress of R. solanacearum infection in Arabidopsis is still unclear. We generated a bioluminescent R. solanacearum by expressing plasmid-based luxCDABE. Expression of luxCDABE did not alter the bacterial growth and pathogenicity. The light intensity of bioluminescent R. solanacearum was linearly related to bacterial concentrations from 104 to 108 CFU·mL−1. After root inoculation with bioluminescent R. solanacearum strain, light signals in tomato and Arabidopsis were found to be transported from roots to stems via the vasculature. Quantification of light intensity from the bioluminescent strain accurately reported the difference in disease resistance between Arabidopsis wild type and resistant mutants. Bioluminescent R. solanacearum strain spatially and quantitatively measured bacterial growth in tomato and Arabidopsis, and offered a tool for the high-throughput study of R. solanacearum-Arabidopsis interaction in the future.