Improvement of efficiency of genetic transformation for Dunaliella salina by glass beads method
Improvement of efficiency of genetic transformation for Dunaliella salina by glass beads method
复制标题
玻璃珠法提高盐生杜氏藻遗传转化效率
DOI：
10.1007/s11033-008-9333-1
复制
发表时间：
2009-07-01
影响因子：
2.8
通讯作者：
Lu, Pengju
中科院分区：
文献类型：
--
作者：
Feng, Shuying;Xue, Lexun;Lu, Pengju
Dunaliella salina has been exploited as a new type of bioreactor due to its unique advantages. However, this bioreactor application was restricted for absence of a high-efficiency and stable transformation method at present. In the present study, the cells of D. salina were transformed by glass beads. The results of histochemical staining revealed that the GUS gene was successfully expressed in the positive transformants, and PCR and PCR-Southern blot analysis further demonstrated that the bar gene was integrated into the D. salina genome. Moreover, the three transformation methods, including glass beads, bombardment particle and electroporation, were compared for screening a high-efficiency transformation method for gene engineering of D. salina. The results showed that transformation efficiency of the glass beads was the highest, approximately 10(2) transformants/microg DNA. It is concluded that the established glass beads method has been demonstrated to be an optimal transformation way for D. salina.
