PLAKOGLOBIN - A PROTEIN COMMON TO DIFFERENT KINDS OF INTERCELLULAR ADHERING JUNCTIONS
PLAKOGLOBIN - A PROTEIN COMMON TO DIFFERENT KINDS OF INTERCELLULAR ADHERING JUNCTIONS
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DOI：
10.1016/0092-8674(86)90706-3
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发表时间：
1986-09-26
期刊：
影响因子：
64.5
通讯作者：
HYNES, RO
中科院分区：
文献类型：
--
作者：
COWIN, P;KAPPRELL, HP;HYNES, RO
我们已经通过单克隆抗体和cDNA克隆确定，Mr为83，000的桥粒多肽也出现在其他类型的粘附连接的斑块上，包括粘着斑蛋白-肌动蛋白相关的细胞间连接，例如，上皮细胞的粘附小带和内皮、透镜和支持细胞连接。这是第一个在其他生物化学上不同的斑块域中发现的共同成分。尽管其浓度在这些细胞间连接处，但在相应的细胞-基质接触部位不存在。此外，它在细胞质中以球形可溶性7S形式出现。我们讨论的意义，这种蛋白质，其中的名称斑珠蛋白的建议，在其相互作用方面与这种生化不同的膜结构域和它们的不同类型的相关的细胞骨架丝。
We have established, by means of a monoclonal antibody and a cDNA clone, that a desmosomal polypeptide of Mr 83,000 also occurs at the plaques of other types of adhering junctions, including the vinculin-actin-associated intercellular junctions, e.g., the zonula adhaerens of epithelial cells and the endothelial, lens, and Sertoli cell junctions. This is the first component found in common among otherwise biochemically distinct plaque domains. Despite its concentration at these intercellular junctions, it is absent from the respective cell-substratum contact sites. In addition, it appears in a globular soluble 7S form in the cytoplasm. We discuss the significance of this protein, for which the name plakoglobin is proposed, in terms of its interaction with such biochemically diverse membrane domains and their different types of associated cytoskeletal filaments.