Characterization of species C human adenovirus serotype 6 (Ad6).
Characterization of species C human adenovirus serotype 6 (Ad6).
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DOI：
10.1016/j.virol.2010.10.041
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发表时间：
2011-03-30
期刊：
影响因子：
3.7
通讯作者：
Barry MA
中科院分区：
文献类型：
--
作者：
Weaver EA;Hillestad ML;Khare R;Palmer D;Ng P;Barry MA
腺病毒血清型（Ad5）是研究最多的一种Ad。Ad1、ad2和ad6也是C Ad物种的成员，并被认为具有与Ad5相似的生物学特性。在这项工作中，我们比较了Ad1、2、5和6在静脉注射和肌肉注射后感染肝脏和肌肉的能力。我们惊奇地发现，Ad6在肝脏基因传递中最有效，而Ad1和Ad2明显弱于Ad5和Ad6。为了了解这些差异，我们对Ad6基因组进行了测序。这表明，Ad6纤维蛋白比其他蛋白惊人地短3个轴重复，这可能解释了体外病毒传染性的差异，但在肝脏中却没有。六正体高变区（HVRs）的比较表明，Ad5和ad6的转导比Ad1和ad2高，可能与电荷和长度的差异有关。
Adenovirus serotype (Ad5) is the most studied Ad. Ad1, 2, and 6 are also members of species C Ad and are presumed to have biologies similar to Ad5. In this work, we have compared the ability of Ad1, 2, 5, and 6 to infect liver and muscle after intravenous and intramuscular injection. We found that Ad6 was surprisingly the most potent at liver gene delivery and that Ad1 and Ad2 were markedly weaker than Ad5 and 6. To understand these differences, we sequenced the Ad6 genome. This revealed that the Ad6 fiber protein is surprisingly three shaft repeats shorter than the others which may explain differences in virus infectivity in vitro, but not in the liver. Comparison of hexon hypervariable regions (HVRs) suggests that the higher transduction by Ad5 and 6 as compared to Ad1 and 2 may be related to differences in charge and length.
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