Detection of cytokeratins 19/20 and guanylyl cyclase C in peripheral blood of colorectal cancer patients
Detection of cytokeratins 19/20 and guanylyl cyclase C in peripheral blood of colorectal cancer patients
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DOI：
10.1038/sj.bjc.6690289
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发表时间：
1999-04-01
影响因子：
8.8
通讯作者：
Dorudi, S
中科院分区：
文献类型：
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作者：
Bustin, SA;Gyselman, VG;Dorudi, S
使用聚合酶链反应（PCR）检测血液中假定的肿瘤细胞来源的mRNA转录本的临床意义尚不清楚。我们使用完全定量的5 '-核酸酶RT-PCR检测21名健康对照和27名结直肠癌患者外周血中细胞角蛋白（ck）19和20以及鸟苷酸环化酶C（GCC）的表达。细胞角蛋白19和20 mRNA的表达分别在30%和100%的样本中检测到，取自健康志愿者。CK 19和CK 20 mRNA转录水平在正常对照组和患者组之间以及不同Dukes分期患者组之间均无明显差异。虽然仅在1/21的对照样品中检测到GCC mRNA，但其在约80%的患者中表达，尽管GCC水平与疾病阶段之间再次没有相关性。所有三个标记的转录水平在样本之间变化很大，甚至在不同时间从同一个人身上采集的样本之间也是如此。我们的结论是，无论是CK 19还是CK 20是可靠的标记物，用于检测外周血中的结肠上皮细胞和GCC的有用性的评价有待进一步的纵向研究。
The clinical significance of detecting supposed tumour cell-derived mRNA transcripts in blood using the polymerase chain reaction (PCR) remains unclear. We have used a fully quantitative 5'-nuclease RT-PCR assay to screen for the expression of cytokeratins (ck) 19 and 20 and guanylyl cyclase C (GCC) in the peripheral blood of 21 healthy controls and 27 colorectal cancer patients. Expression of cytokeratin 19 and 20 mRNA was detected in 30% and 100% of samples, respectively, taken from healthy volunteers. There was no apparent difference in ck19 and ck20 mRNA transcription levels between controls and patients, or between patients with different Dukes' stages. While GCC mRNA was detected in only 1/21 control samples, it was expressed in approximately 80% of patients, although again there was no correlation between GCC levels and disease stage. Transcription levels of all three markers varied considerably between samples, even between samples taken from the same person at different times. We conclude that neither ck19 nor ck20 are reliable markers for the detection of colon epithelial cells in peripheral blood and that an evaluation of the usefulness of GCC awaits further longitudinal studies.