Differentiation of intercalated cells in developing rat kidney: an immunohistochemical study.
Differentiation of intercalated cells in developing rat kidney: an immunohistochemical study.
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发育中的大鼠肾脏中嵌入细胞的分化：一项免疫组织化学研究。
DOI：
10.1152/ajprenal.1994.266.6.f977
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Madsen,KM
中科院分区：
文献类型：
--
作者：
Kim,J;Tisher,CC;Madsen,KM
嵌入细胞存在于源自输尿管芽的集合管和作为肾单位一部分的连接小管 (CNT) 中，因此由后肾胚基发育而来。然而，闰细胞的胚胎学起源尚未确定。 CNT 和皮质集合管 (CCD) 中存在 A 型和 B 型两种闰细胞群。然而，尚不确定这些细胞是否代表真正不同的细胞类型，或者一种细胞是否源自另一种细胞类型。在本研究中，我们使用碳酸酐酶 II (CA II)、H(+)-三磷酸腺苷酶 (H(+)-ATPase) 和带 3 蛋白的特异性抗体来识别嵌入细胞的亚群，确定其出现的部位和时间，并跟踪它们在发育中的大鼠肾脏中的分化。保存 16、17、18 和 20 天胎儿的产前肾脏，以及 0、3、7、14 和 21 天幼仔的产后肾脏用于免疫组织化学研究。 CA II 和 H(+)-ATPase 的免疫染色同时出现在 18 天胎儿 CNT 和髓质集合管 (MCD) 的细胞亚群中，表明插入的细胞从不同的病灶中分化出来，一个位于肾单位，一个位于集合管。妊娠18天时，CNT和MCD中出现顶端细胞和基底外侧H(+)-ATP酶标记细胞，表明A型和B型细胞在肾脏发育过程中同时分化。胎儿肾脏中的带 3 免疫染色非常弱，但在 3 天大的肾脏中观察到标记显着增加，表明出生后不久就会激活泌酸细胞。在胎儿肾脏中，在整个 MCD 和乳头表面的细胞中观察到 CA II 和 H(+)-ATPase 的免疫染色。出生后，乳头表面和末端内侧MCD的免疫染色逐渐消失，基底外侧标记H(+)-ATPase的细胞从外侧MCD逐渐消失。这项研究的结果表明，A 型和 B 型嵌入细胞代表了不同的细胞类型，它们源自两个不同病灶（一个位于肾单位，一个位于集合管）的未分化细胞。我们的结果还表明，在正常肾脏发育过程中，整个嵌入细胞群从集合管中消除。
Intercalated cells are present in both the collecting duct, which is derived from the ureteric bud, and the connecting tubule (CNT), which is part of the nephron and thus is developed from the metanephric blastema. However, the embryologic origin of the intercalated cells has not been established. Two populations of intercalated cells, type A and type B, exist in the CNT and the cortical collecting duct (CCD). It is uncertain, however, whether these cells represent truly distinct cell types or whether one is derived from the other. In this study we have used specific antibodies to carbonic anhydrase II (CA II), H(+)-adenosinetriphosphatase (H(+)-ATPase), and band 3 protein to identify subpopulations of intercalated cells, to determine the site and time of their appearance, and to follow their differentiation in the developing rat kidney. Prenatal kidneys from 16-, 17-, 18-, and 20-day-old fetuses, and postnatal kidneys from 0-, 3-, 7-, 14-, and 21-day-old pups were preserved for immunohistochemical studies. Immunostaining for CA II and H(+)-ATPase appeared simultaneously in a subpopulation of cells in the CNT and the medullary collecting duct (MCD) of the 18-day-old fetus, suggesting that intercalated cells differentiate from separate foci, one in the nephron and one in the collecting duct. Cells with apical and cells with basolateral labeling for H(+)-ATPase appeared in the CNT and MCD at 18 days of gestation, indicating that type A and type B cells differentiate simultaneously during renal development. Band 3 immunostaining was very weak in the fetal kidney, but a striking increase in labeling was observed in the 3-day-old kidney, suggesting that there is an activation of acid-secreting cells shortly after birth. In the fetal kidney, immunostaining for CA II and H(+)-ATPase was observed in cells throughout the MCD and on the papillary surface. After birth, immunostaining gradually disappeared from both the papillary surface and the terminal inner MCD, and cells with basolateral labeling for H(+)-ATPase gradually disappeared from the outer MCD. The results of this study suggest that type A and type B intercalated cells represent distinct cell types that derive from undifferentiated cells at two separate foci, one in the nephron and one in the collecting duct. Our results also suggest that entire populations of intercalated cells are eliminated from the collecting duct during normal renal development.