Stimulation of human cytotoxic T cells with HIV-1-derived peptides presented by recombinant HLA-A2 peptide complexes.
Stimulation of human cytotoxic T cells with HIV-1-derived peptides presented by recombinant HLA-A2 peptide complexes.
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用重组 HLA-A2 肽复合物呈递的 HIV-1 衍生肽刺激人细胞毒性 T 细胞。
DOI：
10.1093/intimm/9.3.451
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发表时间：
1997
影响因子：
4.4
通讯作者：
Eisen,HN
中科院分区：
文献类型：
--
作者：
Walter,JB;Brander,C;Mammen,M;Garboczi,DN;Kalams,SA;Whitesides,GM;Walker,BD;Eisen,HN
HLA-A2重链和β 2-微球蛋白在大肠杆菌中表达，并在来自HIV-1 RT和gag蛋白的肽的存在下重折叠。当重组HLA-A2分子附着于缺乏HLA-A2的细胞时，细胞变得对HLA-A2限制性细胞毒性T淋巴细胞（CTL）克隆的裂解敏感，所述克隆对来自RT和gag蛋白的肽具有特异性。HIV-1感染者外周血单个核细胞的有限稀释分析表明，共价固定在微球上的重组HLA-A2肽复合物刺激HLA-A2肽特异性CTL的发展。预形成的HLA-肽复合物可以提供依赖于抗原的细胞内加工以引发T细胞应答的免疫程序的替代方案。
HLA-A2 heavy chain and beta 2-microglobulin were expressed in Escherichia coli, and refolded in the presence of peptides derived from HIV-1 RT and gag proteins. When recombinant HLA-A2 molecules were attached to cells lacking HLA-A2, the cells became susceptible to lysis by HLA-A2-restricted cytotoxic T lymphocyte (CTL) clones specific for peptides derived from RT and gag proteins. Limiting dilution analyses of peripheral blood mononuclear cells from HIV-1-infected individuals showed that the recombinant HLA-A2 peptide complexes covalently immobilized on microspheres stimulated the development of HLA-A2 peptide-specific CTL. Preformed HLA-peptide complexes may provide an alternative to immunization procedures that depend upon intracellular processing of antigen to elicit T cell responses.