Tectal CRFR1 receptors modulate food intake and feeding behavior in the South African clawed frog Xenopus laevis
Tectal CRFR1 receptors modulate food intake and feeding behavior in the South African clawed frog Xenopus laevis
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DOI：
10.1016/j.yhbeh.2018.07.013
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发表时间：
2018-09-01
影响因子：
3.5
通讯作者：
Carr, James A.
中科院分区：
文献类型：
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作者：
Prater, Christine M.;Harris, Breanna N.;Carr, James A.
当存在视觉威胁时，视顶盖和上丘会迅速抑制食物的摄入。以前的工作表明，CRF作用于CRFR1受体，可能在顶盖抑制摄食行为和食物摄取方面发挥作用。在这里，我们测试了顶盖CRFR1受体调节非洲爪哇幼体摄食和摄食行为的假设。我们进行了五个实验来测试以下问题：1)顶盖注射CRF是否减少了摄食/摄食行为？2)选择性CRFR1拮抗剂是否阻断了CRF对摄食/摄食行为的影响？3)反应性应激源是否减少了摄食/摄食行为？4)选择性CRFR1拮抗剂是否阻断了反应性应激导致的摄食/摄食行为的减少？5)食物剥夺是否增加了摄食/摄食行为？顶盖CRF注射减少了食物的摄入，并影响了探索行为、后肢踢腿和与食物接触的时间。这些作用可被选择性的R1拮抗剂NbI-27914阻断。暴露在反应性应激源下会减少食物摄入量，这种作用可被NBI-27914阻断。在1wk的食物剥夺后，摄食量和摄食行为都没有改变。总体而言，我们得出的结论是，顶盖CRFR1的激活抑制了幼体的摄食量。此外，顶盖CRFR1受体似乎与反应性应激源引起的食物摄入量减少有关。
The optic tectum and superior colliculus rapidly inhibit food intake when a visual threat is present. Previous work indicates that CRF, acting on CRFR1 receptors, may play a role in tectal inhibition of feeding behavior and food intake. Here we test the hypothesis that tectal CRFR1 receptors modulate food intake and feeding behavior in juvenile Xenopus Iaevis. We performed five experiments to test the following questions: 1) Does tectal CRF injection decrease food intake/feeding behavior? 2) Does a selective CRFR1 antagonist block CRF effects on feeding/feeding behavior? 3) Does a reactive stressor decrease food intake/feeding behavior? 4) Does a selective CRFR1 antagonist block reactive stress-induced decrease in feeding/feeding behavior? 5) Does food deprivation increase food intake/feeding behavior? Tectal CRF injections reduced food intake and influenced exploratory behavior, hindlimb kicks, and time in contact with food. These effects were blocked by the selective R1 antagonist NBI-27914. Exposure to a reactive stressor decreased food intake and this effect was blocked by NBI-27914. Neither food intake or feeding behavior changed following 1 wk of food deprivation. Overall, we conclude that activation of tectal CRFR1 inhibits food intake in juvenile X. laevis. Furthermore, tectal CRFR1 receptors appear to be involved in the reduction of food intake that occurs in response to a reactive stressor.