Cortical Constriction During Abscission Involves Helices of ESCRT-III-Dependent Filaments
Cortical Constriction During Abscission Involves Helices of ESCRT-III-Dependent Filaments
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DOI：
10.1126/science.1201847
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发表时间：
2011-03-25
期刊：
影响因子：
56.9
通讯作者：
Gerlich, Daniel W.
中科院分区：
文献类型：
--
作者：
Guizetti, Julien;Schermelleh, Lothar;Gerlich, Daniel W.
细胞质内容物通过卵裂沟内移进行分配后，动物细胞通过细胞间桥保持连接，随后通过分裂而分裂。在这里，我们研究了中间阶段的人类细胞的凋亡，通过使用实时成像，三维结构照明显微镜，和电子断层扫描。我们确定了17纳米直径的螺旋细丝，它将细胞间桥的皮质缩小到一个单一的柄。运输所需的内体分选复合物（ESCRT）-III与收缩区共定位，并需要组装17纳米直径的细丝。同时spastin介导的底层微管的去除，使完全收缩在pancreission网站。在细胞间桥的收缩丝螺旋的识别具有广泛的意义，为理解细胞分裂和ESCRT-III介导的大膜结构的裂变。
After partitioning of cytoplasmic contents by cleavage furrow ingression, animal cells remain connected by an intercellular bridge, which subsequently splits by abscission. Here, we examined intermediate stages of abscission in human cells by using live imaging, three-dimensional structured illumination microscopy, and electron tomography. We identified helices of 17-nanometer-diameter filaments, which narrowed the cortex of the intercellular bridge to a single stalk. The endosomal sorting complex required for transport (ESCRT)-III co-localized with constriction zones and was required for assembly of 17-nanometer-diameter filaments. Simultaneous spastin-mediated removal of underlying microtubules enabled full constriction at the abscission site. The identification of contractile filament helices at the intercellular bridge has broad implications for the understanding of cell division and of ESCRT-III-mediated fission of large membrane structures.