PCK1 expression is correlated with the plasma glucose level in the duck
PCK1 expression is correlated with the plasma glucose level in the duck
复制标题
PCK1 表达与鸭的血浆葡萄糖水平相关
DOI：
10.1111/age.12540
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发表时间：
2017-06-01
期刊：
影响因子：
2.4
通讯作者：
Lu, L. Z.
中科院分区：
文献类型：
--
作者：
Chen, L.;Zeng, T.;Lu, L. Z.
磷酸烯醇式丙酮酸羧激酶1（可溶性）（PCK 1）是甘油合成和甘油生成的关键基因。虽然它的功能已经在老鼠、蝙蝠和人类身上进行了广泛的研究，但对鸭子的研究却知之甚少。在这里，PCK 1的功能进行了研究，采用鸭驯化模型和48小时禁食实验。结果表明，缙云麻鸭和樱桃谷鸭PCK 1的表达量显著低于野鸭。同时，家鸭血浆葡萄糖、甘油三酯和游离脂肪酸水平低于野鸭。与饲喂鸭相比，血浆甘油三酯水平显着降低，而葡萄糖和游离脂肪酸水平保持不变，在48小时禁食鸭。促凋亡基因的表达分析显示，果糖-1，6-二磷酸酶基因（FBP 1和FBP 2）和葡萄糖-6-磷酸酶基因（G6 PC 2）没有改变，而PCK 1显著上调。此外，已报道的PCK 1调控基因FOXA 2和NR 4A家族基因NR 4A 1、NR 4A 2和NR 4A 3在禁食48 h鸭和饲喂鸭中的表达水平相似，表明PCK 1在禁食条件下不受这些基因的调控。因此，PCK 1的表达可能会影响鸭驯化过程中血浆葡萄糖、甘油三酯和游离脂肪酸的水平。这项工作首次在鸭中证明了PCK 1是维持禁食期间血浆葡萄糖稳态的关键基因，并且PCK 1的上调表达可能是通过甘油生成过程维持血浆游离脂肪酸水平恒定的原因。
Phosphoenolpyruvate carboxykinase 1 (soluble) (PCK1) is a key gene in gluconeogenesis and glyceroneogenesis. Although its functions have been extensively studied in mice, bats and humans, little is known in ducks. Here, PCK1 functions were studied using a duck domestication model and a 48-h fasting experiment. We found PCK1 expression significantly decreased in two breeds of domestic ducks (Jinyun Pockmark ducks and Cherry Valley ducks) as compared with wild ducks (Anas platyrhynchos). Simultaneously, plasma levels of glucose, triglycerides and free fatty acid in domestic ducks were lower than in wild ducks. When compared with fed ducks, the plasma triglyceride level was observed to be significantly decreased, while the glucose and free fatty acid levels remained constant in 48-h fasting ducks. The expression analysis of gluconeogenic genes revealed that fructose-1,6-bisphosphatase genes (FBP1 and FBP2) and the glucose-6-phosphatase gene (G6PC2) were not changed, whereas PCK1 was significantly upregulated. In addition, the reported regulators of PCK1, including forkhead box A2 (FOXA2) gene and orphan nuclear receptor NR4A family genes (NR4A1, NR4A2 and NR4A3), exhibited similar expression levels between 48-h fasting ducks and fed ducks, suggesting that PCK1 is not regulated by these genes in the duck under fasting conditions. In conclusion, PCK1 expression may affect plasma levels of glucose, triglycerides and free fatty acid during the duck domestication process. This work demonstrates for the first time in duck that PCK1 is a key gene in maintaining plasma glucose homeostasis during fasting and that the upregulated expression of PCK1 may be responsible for constant plasma free fatty acid level by the glyceroneogenesis process.