Gene Transfer and Living Release of Transforming Growth Factor-β3 for Cartilage Tissue Engineering Applications
Gene Transfer and Living Release of Transforming Growth Factor-β3 for Cartilage Tissue Engineering Applications
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DOI：
10.1089/ten.tec.2008.0163
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发表时间：
2008-12-01
影响因子：
3
通讯作者：
Wang, Dong-an
中科院分区：
文献类型：
--
作者：
Hao, Jinghua;Yao, Yongchang;Wang, Dong-an
由于软骨的非血管性质，软骨修复继续呈现巨大的临床挑战。许多研究表明，通过人工剂量的生长因子可以在体外实现祖细胞的软骨形成；然而，如何在体内为植入的治疗细胞提供生长因子仍然是一个重要的难题。本研究构建了编码人转化生长因子- β 3 （htgf - β 3）的重组腺病毒，并将其应用于大鼠骨髓间充质基质细胞和关节软骨细胞，用于软骨再生。获得了最佳可行转导和转基因htgf - β 3的产生；因此，在受感染的祖细胞中，软骨标记物- II型胶原蛋白的阳性表达得以实现。因此，我们认为编码tgf - β 3基因的重组腺病毒已经成功建立并验证了其在软骨组织工程中的应用。
Cartilage restoration continues to present a tremendous clinical challenge due to its nonvascular nature. Many studies have demonstrated that chondrogenesis of progenitor cells can be achieved in vitro by manual dose of growth factors; however, it remains a vital difficulty in feeding growth factors to implanted therapeutic cells in vivo. Herein, we constructed recombinant adenovirus encoding human transforming growth factor-beta 3 (hTGF-beta 3) and practiced it in rat bone marrow-derived mesenchymal stromal cells and articular chondrocytes for cartilage regeneration. Optimal viable transduction and transgenic hTGF-beta 3 production were achieved; consequently, positive expression of cartilage marker-collagen type II was enabled in the infected progenitors. We thus conclude that recombinant adenovirus encoding TGF-beta 3 gene has been successfully established and validated for cartilage tissue engineering applications.