Cryptic occurrence of Chattonella marina var. marina in mangrove sediments in Probolinggo, East Java Province, Indonesia
Cryptic occurrence of Chattonella marina var. marina in mangrove sediments in Probolinggo, East Java Province, Indonesia
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DOI：
10.1007/s12562-018-1219-0
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发表时间：
2018-06
影响因子：
1.9
通讯作者：
Ayu-Lana-Nafisyah;Endang-Dewi-Masithah;K. Matsuoka;Mirni-Lamid;Mochammad-Amin-Alamsjah;Shizuka O-Hara;K. Koike
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文献类型：
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作者：
Ayu-Lana-Nafisyah;Endang-Dewi-Masithah;K. Matsuoka;Mirni-Lamid;Mochammad-Amin-Alamsjah;Shizuka O-Hara;K. Koike
众所周知，红树林和邻近的小溪是高产的河口，它们部分由生长在沉积物上的底栖微藻支撑。在对爪哇岛东部红树林沼泽的微藻区系进行调查时，一份泥浆样本出人意料地包含了大量臭名昭著的鱼类杀手——类似查顿奈菌的活动细胞及其静止的囊。将这些运动细胞作为克隆培养物进行进一步鉴定和生理试验。通过孢粉学和分子生物学手段对囊肿进行了检查。光镜鉴定和核糖体RNA基因序列鉴定证实这些细胞和囊肿为马氏卡顿菌。虽然菌株与从日本和中国分离的温带菌株在遗传上相同，但温度实验表明，即使暴露在34°C（日本菌株无法存活的温度）下，印度尼西亚菌株也具有较高的光系统II的最大量子产率。盐度试验表明，该菌株对盐度为15的环境具有适应性。这些发现，连同在红树林沉积物中发现的囊孢种群，突出了印度尼西亚菌株的坚韧和独特性质，它们可能适应红树林沼泽温度和盐度的大幅度波动，并对印度尼西亚的渔业构成潜在风险。
Mangrove forests and adjacent creeks are known to be highly productive estuaries, which are partly supported by benthic microalgae that grow on the sediments. During surveys investigating the microalgal floras of mangrove swamps in the eastern part of Java Island, a mud sample unexpectedly included large numbers of a notorious fish killer,Chattonella-like motile cells, and its resting cysts. These motile cells were established as clonal cultures for further identification and physiological tests. The cysts were examined through palynological and molecular biological means. Identification based on light microscopy and ribosomal RNA gene sequences confirmed that these cells and cysts wereChattonella marinavar.marina. While the strains were genetically identical to the temperate strains isolated from Japan and China, temperature experiments showed that the Indonesian strains possessed a high maximum quantum yield of photosystem II even after exposure to 34 °C, a temperature at which the Japanese strain could not survive. Salinity experiments showed adaptation of the strains to a salinity of 15. These findings, together with the discovery of populations of cysts in the mangrove sediment, highlight the tough and unique nature of the Indonesian strains, which are likely adapted to wide fluctuations of temperature and salinity in mangrove swamps, and pose a potential risk to fisheries in Indonesia.