HEPATOCYTE NUCLEAR FACTOR-3-ALPHA BELONGS TO A GENE FAMILY IN MAMMALS THAT IS HOMOLOGOUS TO THE DROSOPHILA HOMEOTIC GENE FORK-HEAD
HEPATOCYTE NUCLEAR FACTOR-3-ALPHA BELONGS TO A GENE FAMILY IN MAMMALS THAT IS HOMOLOGOUS TO THE DROSOPHILA HOMEOTIC GENE FORK-HEAD
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DOI：
10.1101/gad.5.3.416
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发表时间：
1991-03-01
影响因子：
10.5
通讯作者：
DARNELL, JE
中科院分区：
文献类型：
--
作者：
LAI, E;PREZIOSO, VR;DARNELL, JE
通过分析与编码最近描述的大鼠蛋白肝细胞核因子3- α （hnf -3- α，以前称为HNF-3A）的部分cDNA交叉杂交的cDNA克隆，我们现在描述了该基因家族的两个额外成员，hnf -3- β和hnf -3- γ。这个家族的dna结合域有一个110个氨基酸的区域，不仅在啮齿类动物中高度保守（hnf -3- α、-3- β和-3- γ在该区域110个氨基酸中有93个氨基酸相同），而且在果蝇中也是如此，同源基因叉头在3个大鼠基因中93个残基中有88个是相同的。在啮齿类动物中，HNF-3家族在源自原始肠道内壁的细胞中表达；一些表达叉头的胚胎果蝇细胞也会发育成肠腺和唾液腺。因此，这个基因家族，其dna结合部分不同于任何先前已知的dna结合蛋白，可能有助于脊椎动物和无脊椎动物内脏器官细胞的分化。
By analysis of cDNA clones that cross-hybridized with a portion of the cDNA encoding the recently described rat protein hepatocyte nuclear factor 3-alpha (HNF-3-alpha, previously called HNF-3A), we now describe two additional members, HNF-3-beta and HNF-3-gamma, of this gene family. A 110-amino-acid region in the DNA-binding domain of this family is not only very highly conserved in rodents (HNF-3-alpha, -3-beta, and -3-gamma are identical in 93 of 110 amino acids in this region) but also in Drosophila where the homeotic gene fork head has 88 of the 93 residues that are identical in the three rat genes. The HNF-3 family in rodents is expressed in cells that derive from the lining of the primitive gut; some of the embryonic Drosophila cells in which fork head is expressed also give rise to gut and salivary glands. Thus, it appears that this gene family, the DNA-binding portion of which is unlike that of any previously recognized DNA-binding proteins, may contribute to differentiation of cells in internal organs in both vertebrates and invertebrates.