Cotton metallothionein GhMT3a, a reactive oxygen species scavenger, increased tolerance against abiotic stress in transgenic tobacco and yeast.
Cotton metallothionein GhMT3a, a reactive oxygen species scavenger, increased tolerance against abiotic stress in transgenic tobacco and yeast.
复制标题
棉花金属硫蛋白 GhMT3a（一种活性氧清除剂）可提高转基因烟草和酵母对非生物胁迫的耐受性
DOI：
10.1093/jxb/ern291
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发表时间：
2009
影响因子：
6.9
通讯作者：
Zheng C
中科院分区：
文献类型：
--
作者：
Xue T;Li X;Zhu W;Wu C;Yang G;Zheng C
通过cDNA文库筛选，从棉花（Gossypium hirsutum）中克隆了一个编码64个氨基酸的3型金属硫蛋白（MT3a）的cDNA克隆。北方印迹分析表明，棉花幼苗GhMT 3a mRNA的积累不仅受到高盐、干旱和低温胁迫的影响，而且受到重金属离子、脱落酸（ABA）、乙烯和活性氧（ROS）的影响。与野生型植物相比，过表达GhMT3a的转基因烟草（Nicotiana tabacum）植物对非生物胁迫表现出增加的耐受性。有趣的是，在抗氧化剂的存在下，盐，干旱和低温胁迫诱导的GhMT3a的表达可以被抑制。在这种胁迫条件下，转基因烟草植株中的H2O2水平仅为野生型（WT）植株中的一半。体外实验表明，重组GhMT 3a蛋白具有与金属离子和ROS结合的能力。过表达GhMT3a的转基因酵母也表现出对ROS胁迫的更高耐受性。综上所述，这些结果表明，GhMT3a可以作为一个有效的ROS清除剂，其表达可以通过ROS信号转导的非生物胁迫进行调节。
A cDNA clone encoding a 64-amino acid type 3 metallothionein protein, designated GhMT3a, was isolated from cotton (Gossypium hirsutum) by cDNA library screening. Northern blot analysis indicated that mRNA accumulation of GhMT3a was up-regulated not only by high salinity, drought, and low temperature stresses, but also by heavy metal ions, abscisic acid (ABA), ethylene, and reactive oxygen species (ROS) in cotton seedlings. Transgenic tobacco (Nicotiana tabacum) plants overexpressing GhMT3a showed increased tolerance against abiotic stresses compared with wild-type plants. Interestingly, the induced expression of GhMT3a by salt, drought, and low-temperature stresses could be inhibited in the presence of antioxidants. H2O2 levels in transgenic tobacco plants were only half of that in wild-type (WT) plants under such stress conditions. According to in vitro assay, recombinant GhMT3a protein showed an ability to bind metal ions and scavenge ROS. Transgenic yeast overexpressing GhMT3a also showed higher tolerance against ROS stresses. Taken together, these results indicated that GhMT3a could function as an effective ROS scavenger and its expression could be regulated by abiotic stresses through ROS signalling.
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影响因子：
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