Bidirectional multisite seizure propagation in the intact isolated hippocampus: The multifocality of the seizure "focus"
Bidirectional multisite seizure propagation in the intact isolated hippocampus: The multifocality of the seizure "focus"
复制标题
DOI：
10.1016/j.nbd.2006.03.014
复制
发表时间：
2006-08-01
影响因子：
6.1
通讯作者：
Carlen, P. L.
中科院分区：
文献类型：
--
作者：
Derchansky, M.;Rokni, D.;Carlen, P. L.
定位癫痫发作的病灶是困难的，而且常常发现多个部位，这反映了我们对癫痫发作发生和传播的基本机制的认识不足。我们使用多位点电生理记录在两个癫痫发作模型和电压敏感染料成像，时空特征的启动和传播癫痫发作在一个完整的致痫性脑区，孤立的海马。在低镁灌注液中，癫痫发作总是起源于颞区，并沿着隔颞轴向隔区传播。在癫痫发作扩散到海马体后，癫痫发作中的爆发变成了双向的，在不同的频率下具有不同的传播模式。当完整的海马体被分离沿着的septotemporal轴，独立的双向活动中观察到的两半，和区域特异性切割的组织显示，CA 3区是癫痫发作的产生和传播的关键。在第二个癫痫发作模型中，使用隔区和颞区CA3区的局灶性强直刺激，癫痫发作总是起源于刺激部位，随后以不同频率发展双向性，如低镁模型中所述，行为与耦合神经元网络振荡器相容。这些数据为癫痫发作和传播的动态多灶性提供了新的见解，揭示了目前单一癫痫发作“病灶”的概念是复杂的。(c)2006年爱思唯尔公司All rights reserved.
Localizing the seizure focus is difficult and frequently, multiple sites are found. This reflects our poor understanding of the fundamental mechanisms of seizure generation and propagation. We used multisite electrophysiological recordings in two seizure models and voltage-sensitive dye imaging, to spatiotemporally characterize the initiation and propagation of seizures in an intact epileptogenic brain region, the isolated hippocampus. In low-magnesium perfusate, seizures always originated in the temporal region, and propagated along the septotemporal axis to the septal region. After the seizure spread across the hippocampus, the bursts within a seizure became bidirectional, with different propagation patterns at different frequencies. When the intact hippocampus was separated along the septotemporal axis, independent bidirectional activity was observed in the two halves, and region-specific cuts to the tissue reveal that the CA3 region is critical for seizure generation and propagation. In a second seizure model, using focal tetanic stimulation of the septal and temporal CA3 region, seizures always originated at the stimulated site with bidirectionality later developing at different frequencies, as noted in the low magnesium model, behavior compatible with coupled neuronal network oscillators. These data provide novel insights into the dynamic multifocality of seizure onset and propagation, revealing that the current concept of a single seizure "focus" is complex. (c) 2006 Elsevier Inc. All rights reserved.