Contribution of fibronectin-binding protein to pathogenesis of Streptococcus equi ssp. zooepidemicus.
Contribution of fibronectin-binding protein to pathogenesis of Streptococcus equi ssp. zooepidemicus.
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DOI：
10.1111/2049-632x.12029
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发表时间：
2013-04
影响因子：
3.3
通讯作者：
L. Yi;Yang Wang;Zhe Ma;Hui Zhang;Yue Li;Jun-xi Zheng;Yong-chun Yang;Chengping Lu;H. Fan
中科院分区：
文献类型：
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作者：
L. Yi;Yang Wang;Zhe Ma;Hui Zhang;Yue Li;Jun-xi Zheng;Yong-chun Yang;Chengping Lu;H. Fan
马链球菌亚种zooprophylmicus（S.动物寄生虫）是许多物种中多种感染的原因。纤连蛋白结合蛋白是细菌细胞表面蛋白，其特异性结合纤连蛋白（FN）。考虑到FN结合蛋白在宿主-病原体相互作用中的特殊作用，我们研究了沙门氏菌FN结合蛋白（FNZ）中一个新的FN结合结构域的功能。小动物。在大肠杆菌中表达了5个重组FNZ基因片段[N1（氨基酸，38-197）、N2（氨基酸，38-603）、N3（氨基酸，41-315）、N4（氨基酸，192-370）和N5（氨基酸，38-225）]，并检测了它们的FN结合活性。结果表明，FNZ氨基端192-225位氨基酸可能与纤维连接蛋白结合。在S.与野生型相比，其导致粘附于HEp-2细胞的能力降低、体内毒力缺陷、生物膜形成降低以及小鼠血液、肝、肺和脾组织中的定殖能力降低。这些结果表明FNZ参与了S.小动物。此外，这项工作提供了一个新的FN结合结构域内FNZ，这将有助于进一步表征S。动物寄生虫FN结合特性。
Streptococcus equi ssp. zooepidemicus (S. zooepidemicus) is responsible for a wide variety of infections in many species. Fibronectin-binding protein is a bacterial cell surface protein, which specifically binds fibronectin (FN). Considering the specific role of FN-binding protein in host-pathogen interactions, we investigated the function of a novel FN-binding domain in the FN-binding protein (FNZ) of S. zooepidemicus. Five recombinant FNZ gene fragments [N1 (amino acids, 38-197), N2 (amino acids, 38-603), N3 (amino acids, 41-315), N4 (amino acids, 192-370), and N5 (amino acids, 38-225)] were expressed in Escherichia coli, and their FN-binding activities were tested. The results showed that amino acids 192-225 in the NH2 -terminal region of FNZ could be responsible for binding fibronectin. The FNZ knockout mutant was constructed in S. zooepidemicus, which results in the reduced capacity to adhere to HEp-2 cell, defective virulence in vivo, decreased biofilm formation, and decreased colonization capacity in blood, liver, lung, and spleen tissues of mice as compared to the wild type. These results suggest that FNZ participates in biofilm formation, FN binding, cell adhesion, and pathogenesis of S. zooepidemicus. Furthermore, this work offers a novel FN-binding domain within FNZ, which will help in further characterization of S. zooepidemicus FN-binding properties.