Standoff Optical Glucose Sensing in Photosynthetic Organisms by a Quantum Dot Fluorescent Probe
Standoff Optical Glucose Sensing in Photosynthetic Organisms by a Quantum Dot Fluorescent Probe
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DOI：
10.1021/acsami.8b07179
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发表时间：
2018-08-29
影响因子：
9.5
通讯作者：
Giraldo, Juan Pablo
中科院分区：
文献类型：
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作者：
Li, Jinming;Wu, Honghong;Giraldo, Juan Pablo
葡萄糖是光合作用的主要产物，也是生物体细胞呼吸的关键能源。在此，我们能够在野生型植物体内光学葡萄糖传感使用量子点（QD）比率法。光学探针由一对QD形成：巯基乙酸封端的QD，其对葡萄糖保持不变（充当内部荧光参考对照），以及硼酸（BA）缀合的QD（BA-QD），其响应于葡萄糖而淬灭其荧光。QD探针的荧光响应在可见光窗口内，其中光合组织具有相对低的背景。它对植物中发现的其他常见糖具有高度选择性，可用于在生理范围内定量植物中高于500 μ M的葡萄糖水平。我们证明，QD荧光探针报告葡萄糖从单个叶绿体藻细胞（轮藻zeylanica）和植物叶组织（拟南芥）在体内通过共聚焦显微镜和一个支架树莓派相机设置。基于量子点的探针表现出明亮的荧光，没有光漂白，可调的发射峰，和植物细胞壁孔隙度下的大小提供了巨大的潜力，在光合生物体中的葡萄糖原位选择性体内监测。
Glucose is a major product of photosynthesis and a key energy source for cellular respiration in organisms. Herein, we enable in vivo optical glucose sensing in wild-type plants using a quantum dot (QD) ratiometric approach. The optical probe is formed by a pair of QDs: thioglycolic acid-capped QDs which remain invariable to glucose (acting as an internal fluorescent reference control) and boronic acid (BA)-conjugated QDs (BA-QD) that quench their fluorescence in response to glucose. The fluorescence response of the QD probe is within the visible light window where photosynthetic tissues have a relatively low background. It is highly selective against other common sugars found in plants and can be used to quantify glucose levels above 500 mu M in planta within the physiological range. We demonstrate that the QD fluorescent probe reports glucose from single chloroplast to algae cells (Chara zeylanica) and plant leaf tissues (Arabidopsis thaliana) in vivo via confocal microscopy and to a standoff Raspberry Pi camera setup. QD-based probes exhibit bright fluorescence, no photobleaching, tunable emission peak, and a size under plant cell wall porosity offering great potential for selective in vivo monitoring of glucose in photosynthetic organisms in situ.