Isolation of human C-reactive protein complementary DNA and localization of the gene to chromosome 1.
Isolation of human C-reactive protein complementary DNA and localization of the gene to chromosome 1.
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分离人 C 反应蛋白互补 DNA 并将该基因定位到 1 号染色体。
DOI：
10.1126/science.6857266
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发表时间：
1983
期刊：
影响因子：
56.9
通讯作者：
D. Woods
中科院分区：
文献类型：
--
作者：
A. Whitehead;G. Bruns;A. Markham;H. Colten;D. Woods
以人工合成的寡核苷酸混合物为探针，从成人肝脏互补DNA文库中分离C反应蛋白的互补DNA克隆。克隆的大小范围从700到1100个碱基对，并通过部分DNA序列分析进行鉴定。将一个互补DNA克隆用作探针，用于与从体细胞杂交体中分离的人-啮齿动物DNA杂交，并结合到硝酸纤维素滤膜上（Southern印迹分析），以将人C-反应蛋白基因分配到1号染色体。
With a synthetic oligonucleotide mixture as probe, complementary DNA clones of C-reactive protein were isolated from an adult human liver complementary DNA library. The clones ranged in size from 700 to 1100 base pairs and were identified by partial DNA sequence analysis. One complementary DNA clone was used as a probe for hybridization with human-rodent DNA's isolated from somatic cell hybrids and bound to nitrocellulose filters (Southern blot analysis) to assign the human C-reactive protein gene to chromosome 1.