Three-dimensional porous hydroxyapatite/collagen composite with rubber-like elasticity
Three-dimensional porous hydroxyapatite/collagen composite with rubber-like elasticity
复制标题
DOI：
10.1163/156856207780425077
复制
发表时间：
2007-01
期刊：
影响因子：
--
通讯作者：
Shunji Yunoki;T. Ikoma;A. Monkawa;E. Marukawa;S. Sotome;K. Shinomiya;J. Tanaka
中科院分区：
文献类型：
--
作者：
Shunji Yunoki;T. Ikoma;A. Monkawa;E. Marukawa;S. Sotome;K. Shinomiya;J. Tanaka
采用冷冻干燥法制备了具有随机孔结构的三维多孔羟基磷灰石/胶原（HAp/Col）复合材料；将重量比为80.5:19.5的自组织HAp/Col纳米复合材料冷冻干燥，在100 mM磷酸钠缓冲液中揉搓，在- 20℃冷冻后冷冻干燥。在真空条件下，在140℃脱水热条件下引入了Col分子的交联反应。多孔复合材料的孔隙率为94.7%，孔径在200 ~ 500 μm之间。湿多孔复合材料在磷酸盐缓冲盐水（PBS）中的压应力在20天的培养过程中逐渐降低，并有少量失重。循环压缩试验和时程压缩试验结果表明，复合材料的应力重复性好，高度恢复良好，且未引起多孔复合材料的坍塌。多孔复合材料在大鼠骨孔内植入后显示出可生物降解的特性，在骨孔内形成新骨，无炎症反应。所制备的多孔复合材料具有良好的柔韧性和橡胶样弹性，是一种很有前途的骨再生材料。
A three-dimensional porous hydroxyapatite/collagen (HAp/Col) composite with a random pore structure was fabricated using freeze-drying processes; the self-organized HAp/Col nanocomposite with a weight ratio of 80.5:19.5, freeze-dried, was kneaded in 100 mM sodium phosphate buffer, frozen at −20°C and freeze-dried. The cross-linkage of Col molecules was introduced dehydrothermally at 140°C in vacuo. The porous composite had a porosity of 94.7% with pore sizes between 200 and 500 μm. The compressive stress for the wet porous composite in phosphate buffer saline (PBS) was gradually decreased during 20 days incubation with a small amount of weight loss. The cyclic and time-course compression tests showed good repeatability of stress and well-recovery of its height, and caused no collapse of the porous composite. The implantation of the porous composite in rat bone holes showed the biodegradable property and new bone formation occurred in the pores without inflammatory response. The porous composite fabricated has good flexibility and rubber-like elasticity, and is a promising bone regenerative material.