Proteomic analysis of seed viability in maize
Proteomic analysis of seed viability in maize
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玉米种子活力的蛋白质组学分析
DOI：
10.1007/s11738-010-0536-4
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发表时间：
2011-01-01
影响因子：
2.6
通讯作者：
Li, Chaohai
中科院分区：
文献类型：
--
作者：
Wu, Xiaolin;Liu, Haiyan;Li, Chaohai
为了鉴定与玉米种子活力相关的特定蛋白质，基于氯化三苯基四唑（TTC）测定的活力评价对郑单958（中国高产玉米杂交种之一）的种子进行分类，并用于比较蛋白质组分析。 TTC染色后，高活力种子的胚呈深红色（R型），而死种子的胚呈白色（W型）。蛋白质组学分析显示，鉴定出的 28 个蛋白点在 R 和 W 胚胎中表达显着差异，其中 20 个在 R 胚胎中上调，8 个下调。其中包括参与应激反应、蛋白质折叠和稳定的蛋白质，以及与营养储存和代谢相关的蛋白质。值得注意的是，与 W 胚胎相比，R 胚胎中的小热休克蛋白、晚期胚胎发生丰富 (LEA) 蛋白和抗氧化酶高度上调，而两种蛋白酶高度下调。 EMB564 是 LEA 蛋白之一，在种子人工老化过程中其丰度下降。我们的结果表明 EMB564 与玉米种子活力相关。使用这些小蛋白质来开发种子质量的快速测试将会很有趣。
To identify specific proteins related to maize seed viability, seeds of Zhengdan 958 (one of the high-yield maize hybrids in China) were sorted based on viability evaluation with triphenyltetrazolium chloride (TTC) assay and used for comparative proteomic analysis. After TTC staining, embryos of high-viability seeds were deep red (R type), while embryos of dead seeds were white (W type). Proteomic analysis revealed that 28 protein spots identified were differently expressed significantly between R and W embryos, of which 20 were up-regulated and 8 down-regulated in R embryos. Among them were proteins involved in stress response, protein folding, and stabilization, as wells as proteins related to nutrient reservoir and metabolism. Prominently, small heat shock proteins, late embryogenesis abundant (LEA) proteins, and antioxidant enzymes were highly up-regulated, while two proteases were highly down-regulated in R embryos compared to W embryos. One of LEA proteins was EMB564, which declined in abundance during artificial aging of seeds. Our results suggested an association of EMB564 with maize seed viability. It would be of interest to use these small proteins to develop quick tests for seed quality.