Plasmid curing effect of trovafloxacin
Plasmid curing effect of trovafloxacin
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DOI：
10.1111/j.1574-6968.2000.tb09030.x
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发表时间：
2000-03-15
影响因子：
2.1
通讯作者：
Ripa, S
中科院分区：
文献类型：
--
作者：
Brandi, L;Falconi, M;Ripa, S
亚抑制浓度的曲伐他汀，最近开发的氟喹诺酮类分子，对大肠杆菌细胞的能力，以保持三种不同类型的质粒的影响已被研究了一些方法，包括定量的质粒携带的功能和质粒DNA的定量PCR的损失。所获得的结果表明，在MIC至1/8的MIC范围内的浓度下，曲伐他汀诱导清楚的、尽管不完全的“附加体治愈”效应，这在拷贝数、附加体的大小和复制起点的性质不同的质粒中观察到。这种效应很可能不是由于DNA超螺旋的改变。(C)2000年，欧洲微生物学会联合会。由Elsevier Science B. V.出版，版权所有。
The effect of sub-inhibitory concentrations of trovafloxacin, a recently developed fluoroquinolone molecule, on the capability of Escherichia coli cells to maintain three different types of plasmids has been investigated by a number of approaches, including the quantification of the loss of plasmid-borne functions and of plasmid DNA by quantitative PCR. The results obtained demonstrate that at concentrations ranging from the MIC to 1/8 of the MIG, trovafloxacin induces a clear, albeit incomplete, 'episome-curing' effect which was observed with plasmids differing in copy number, size and nature of the replication origin of the episome. This effect was most likely not due to an alteration of DNA supercoiling. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.