The assessment of cold atmospheric plasma treatment of DNA in synthetic models of tissue fluid, tissue and cells
The assessment of cold atmospheric plasma treatment of DNA in synthetic models of tissue fluid, tissue and cells
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DOI：
10.1088/1361-6463/aa7501
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发表时间：
2017-06
期刊：
影响因子：
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通讯作者：
E. Szili;Nishtha Gaur;Sung-Ha Hong;H. Kurita;Jun‐Seok Oh;Masafumi Ito;A. Mizuno;A. Hatta;A. Cowin;D. Graves;R. Short
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文献类型：
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作者：
E. Szili;Nishtha Gaur;Sung-Ha Hong;H. Kurita;Jun‐Seok Oh;Masafumi Ito;A. Mizuno;A. Hatta;A. Cowin;D. Graves;R. Short
有越来越多的文献数据库证明了冷大气等离子体（本文称为等离子体）的治疗潜力。考虑到拟议应用的广度（例如，从牙齿美白到癌症治疗）和正在研究的等离子体设备的广泛范围，更详细地考虑等离子体与细胞特定成分的相互作用是及时的。等离子体可以产生高活性的氧和氮物质（RONS），如羟基自由基（OH·），过氧亚硝酸盐（ONOO−）和超氧化物（O2−），这些物质很容易修饰DNA等基本生物分子。这些修改原则上可以驱动广泛的生物过程。针对这种可能性，所报道的等离子体的治疗作用并没有得到对潜在的等离子体-组织、-细胞或-生物分子相互作用的特别深入的了解的支持。在这项研究中，我们的目标是通过开发简单的模型来研究血浆与DNA的相互作用，以DNA链断裂的形式来部分解决这个问题。这是使用组织液、组织和细胞的合成模型进行的。我们认为，与使用真实的生物材料和细胞相比，这种方法使实验更简单，更具成本效益和更快。在此，氦等离子体射流源被用于这些实验。我们表明，等离子射流很容易诱导DNA链断裂的组织液模型和细胞模型中，令人惊讶的是没有任何显着的穿孔或破裂的磷脂膜。在组织模型的等离子体射流处理中，在延长处理（在分钟的时间尺度上）后在组织块中检测到DNA链断裂，而在组织模型下方的组织液模型中未检测到DNA链断裂。这些数据在血浆的治疗潜力的背景下进行了讨论。
There is a growing literature database that demonstrates the therapeutic potential of cold atmospheric plasma (herein referred to as plasma). Given the breadth of proposed applications (e.g. from teeth whitening to cancer therapy) and vast gamut of plasma devices being researched, it is timely to consider plasma interactions with specific components of the cell in more detail. Plasma can produce highly reactive oxygen and nitrogen species (RONS) such as the hydroxyl radical (OH•), peroxynitrite (ONOO−) and superoxide (O2−) that would readily modify essential biomolecules such as DNA. These modifications could in principle drive a wide range of biological processes. Against this possibility, the reported therapeutic action of plasmas are not underpinned by a particularly deep knowledge of the potential plasma-tissue, -cell or -biomolecule interactions. In this study, we aim to partly address this issue by developing simple models to study plasma interactions with DNA, in the form of DNA-strand breaks. This is carried out using synthetic models of tissue fluid, tissue and cells. We argue that this approach makes experimentation simpler, more cost-effective and faster than compared to working with real biological materials and cells. Herein, a helium plasma jet source was utilised for these experiments. We show that the plasma jet readily induced DNA-strand breaks in the tissue fluid model and in the cell model, surprisingly without any significant poration or rupture of the phospholipid membrane. In the plasma jet treatment of the tissue model, DNA-strand breaks were detected in the tissue mass after pro-longed treatment (on the time-scale of minutes) with no DNA-strand breaks being detected in the tissue fluid model underneath the tissue model. These data are discussed in the context of the therapeutic potential of plasma.