Pathways for compartmentalizing protein synthesis in eukaryotic cells: the template-partitioning model.
Pathways for compartmentalizing protein synthesis in eukaryotic cells: the template-partitioning model.
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真核细胞中蛋白质合成的区室化途径：模板分配模型。
DOI：
10.1139/o05-147
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Pyhtila,Brook
中科院分区：
文献类型：
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作者：
Nicchitta,ChristopherV;Lerner,RachelS;Stephens,SamuelB;Dodd,RebeccaD;Pyhtila,Brook
编码信号序列的mRNAs翻译在内质网结合的核糖体上，而编码胞质蛋白的mRNAs翻译在胞质核糖体上。通过正选择将mRNAs分配到内质网；参与信号序列承载蛋白翻译的胞质核糖体通过信号识别粒子(SRP)途径参与，随后被输送到内质网。研究表明，除了SRP途径外，编码胞浆蛋白的mRNAs也可以被分配给内质网，这表明真核细胞中的RNA分配是一个复杂的过程，需要多个RNA分配途径的活性。本文讨论了这一课题的主要发现，并提出了模板分配模型，该模型描述了真核细胞中RNA分配的假设机制。关键词：mRNA、核糖体、内质网、翻译、蛋白质合成、信号序列、RNA定位。
mRNAs encoding signal sequences are translated on endoplasmic reticulum (ER) - bound ribosomes, whereas mRNAs encoding cytosolic proteins are translated on cytosolic ribosomes. The partitioning of mRNAs to the ER occurs by positive selection; cytosolic ribosomes engaged in the translation of signal-sequence-bearing proteins are engaged by the signal-recognition particle (SRP) pathway and subsequently trafficked to the ER. Studies have demonstrated that, in addition to the SRP pathway, mRNAs encoding cytosolic proteins can also be partitioned to the ER, suggesting that RNA partitioning in the eukaryotic cell is a complex process requiring the activity of multiple RNA-partitioning pathways. In this review, key findings on this topic are discussed, and the template-partitioning model, describing a hypothetical mechanism for RNA partitioning in the eukaryotic cell, is proposed.Key words:mRNA, ribosome, endoplasmic reticulum, translation, protein synthesis, signal sequence, RNA localization.