Simplified quantification of Pittsburgh Compound B amyloid imaging PET studies: a comparative analysis.
Simplified quantification of Pittsburgh Compound B amyloid imaging PET studies: a comparative analysis.
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2005-12
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通讯作者：
B. Lopresti;W. Klunk;C. Mathis;J. Hoge;S. Ziolko;Xueling Lu;C. Meltzer;Kurt Schimmel;N. Tsopelas;S. DeKosky;J. Price
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作者：
B. Lopresti;W. Klunk;C. Mathis;J. Hoge;S. Ziolko;Xueling Lu;C. Meltzer;Kurt Schimmel;N. Tsopelas;S. DeKosky;J. Price
使用淀粉样蛋白结合放射性示踪剂匹兹堡化合物B（PI B）进行了未标记PET研究。先前使用动脉血的定量分析表明，使用90分钟发射数据（ART 90）的Logan图形分析提供了PIB保留的可靠测量。这项工作报告简化的方法分析人类PIB成像。方法对24例Alzheimer病（AD）患者（6例）、轻度认知功能障碍（MCI）患者（10例）、正常对照者（8例）进行PIB PET扫描。8例受试者（AD 3例，MCI 1例，对照4例）在28 d内进行复查扫描。使用Logan分析和（a）基于动脉血浆的代谢物校正输入函数（ART 60、ART 90）、（B）具有群体平均代谢物校正的颈动脉时间-活性数据（CAR 60、CAR 90）和（c）小脑参考组织（CER 60、CER 90）分析60和90 min内的数据。还使用简化的参考组织方法（SRTM 60，SRTM 90）和基于标准化摄取值的后期扫描比率的单次扫描方法（SUVR 60，SUVR 90）分析数据。结果所有的分析方法都有效地检测了AD和对照受试者在淀粉样蛋白负载皮质区域的区域差异，尽管简化方法的性能在偏倚、重测变异性、受试者间变异性和效应量方面有所不同。CAR 90与ART 90分布体积比（DVR）测量在脑区域和受试者组中最一致，并表现出令人满意的重测变异性（跨区域+/-7.1%）。在高DVR受试者中，CER 90和CER 60相对于ART 90显示出负偏倚，但具有最低的重测变异性。基于单次扫描SUV的方法显示出AD和对照组差异的最大效应量，并且在受试者间和重测变异性方面表现良好。结论：在检查的PIB分析的简化方法中，CAR 90提供的DVR测量与ART 90最具可比性; CER 90的重现性最好，SUVR 90产生的效应量最大。所有简化方法均能有效区分AD和对照组的差异，可有效用于PIB的分析。SUVR 60数据可以在短至20分钟的PET发射数据收集中获得。每种实验设计必须考虑每种方法的相对优势和局限性。
UNLABELLED PET studies have been performed using the amyloid binding radiotracer Pittsburgh Compound B (PIB). Previous quantitative analyses using arterial blood showed that the Logan graphical analysis using 90 min of emission data (ART90) provided a reliable measure of PIB retention. This work reports on simplified methods of analysis for human PIB imaging. METHODS PIB PET scans were conducted in 24 subjects (6 Alzheimer's disease [AD], 10 mild cognitive impairment [MCI], 8 controls) with arterial blood sampling. Retest scans were performed on 8 subjects (3 AD, 1 MCI, 4 controls) within 28 d. Data were analyzed over 60 and 90 min using the Logan analysis and (a) metabolite-corrected input functions based on arterial plasma (ART60, ART90), (b) carotid artery time-activity data with a population average metabolite correction (CAR60, CAR90); and (c) cerebellar reference tissue (CER60, CER90). Data also were analyzed using the simplified reference tissue method (SRTM60, SRTM90) and a single-scan method based on late-scan ratios of standardized uptake values (SUVR60, SUVR90). RESULTS All methods of analysis examined effectively discerned regional differences between AD and control subjects in amyloid-laden cortical regions, although the performance of the simplified methods varied in terms of bias, test-retest variability, intersubject variability, and effect size. CAR90 best agreed with ART90 distribution volume ratio (DVR) measures across brain regions and subject groups and demonstrated satisfactory test-retest variability (+/-7.1% across regions). CER90 and CER60 showed negative biases relative to ART90 in high-DVR subjects but had the lowest test-retest variability. The single-scan SUV-based methods showed the largest effect sizes for AD and control group differences and performed well in terms of intersubject and test-retest variability. CONCLUSION Of the simplified methods for PIB analysis examined, CAR90 provided DVR measures that were most comparable to ART90; CER90 was the most reproducible and SUVR90 produced the largest effect size. All simplified methods were effective at distinguishing AD and control differences and may be effectively used in the analysis of PIB. SUVR60 data can be obtained with as little as 20 min of PET emission data collection. The relative strengths and limitations of each method must be considered for each experimental design.