Precision and functional specificity in mRNA decay
Precision and functional specificity in mRNA decay
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DOI：
10.1073/pnas.092538799
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发表时间：
2002-04-30
影响因子：
11.1
通讯作者：
Brown, PO
中科院分区：
文献类型：
--
作者：
Wang, YL;Liu, CL;Brown, PO
mRNA的转录后加工是基因表达程序的一个组成部分。通过使用DNA微阵列，我们精确地测量了温度敏感的RNA聚合酶II热失活后每个酵母mRNA的衰变。半衰期变化很大，从3分钟到90分钟以上不等。我们发现mRNA的半衰期与ORF大小、密码子偏倚、核糖体密度或丰度之间没有简单的相关性。然而，编码在化学计量复合体中一起起作用的蛋白质组的mRNA的衰减率通常是密切匹配的，其他证据表明生理功能和mRNA周转率之间存在更普遍的关系。结果提供了强有力的证据，精确控制每个mRNA的衰变是酵母基因表达程序的基本特征。
Posttranscriptional processing of mRNA is an integral component of the gene expression program. By using DNA microarrays, we precisely measured the decay of each yeast mRNA, after thermal inactivation of a temperature-sensitive RNA polymerase II. The half-lives varied widely, ranging from similar to3 min to more than 90 min. We found no simple correlation between mRNA half-lives and ORF size, codon bias, ribosome density, or abundance. However, the decay rates of mRNAs encoding groups of proteins that act together in stoichiometric complexes were generally closely matched, and other evidence pointed to a more general relationship between physiological function and mRNA turnover rates. The results provide strong evidence that precise control of the decay of each mRNA is a fundamental feature of the gene expression program in yeast.