microRNA-1297 involves in the progression of oral squamous cell carcinoma through PTEN
microRNA-1297 involves in the progression of oral squamous cell carcinoma through PTEN
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DOI：
10.1016/j.sjbs.2018.01.013
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发表时间：
2018-07-01
影响因子：
4.4
通讯作者：
Wei, Bo
中科院分区：
文献类型：
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作者：
Liang, Limin;Feng, Lin;Wei, Bo
背景：口腔鳞状细胞癌（OSCC）的致瘤机制至今仍不清楚。为此，我们拟对一种新发现的OSCC miRNA的生物学作用进行研究。采用TaqManqRT-PCR方法检测16例肿瘤组织中miRNA和基因的表达。CCK-8法和集落形成法检测细胞增殖情况。通过蛋白质印迹法测定蛋白质水平。结果：在口腔鳞癌中，miR-1297被抑制，而PTEN被激活。此外，过表达miR-1297的细胞系显示细胞生长速率降低。而且，抑制miR-1297逆转了细胞生长速率。结论在口腔鳞癌中，miRNA-1297基因的表达改变是一个普遍现象。我们的研究结果表明miR 1297可能通过PTEN驱动肿瘤进展。而且，miR-1297可能是未来研究的一个有希望的候选者。(C)2018年由Elsevier B. V.代表沙特国王大学制作和主持。
Background: Oral squamous cell carcinoma (OSCC) oncogenic mechanisms still remain elusive. Herein, we proposed to understand the biological role of a newly discovered OSCC miRNA.Methods: MiR-1297 related to OSCC was selected for this study. TaqMan qRT- PCR assay was used to profile miRNA and gene expression in 16 tumors with matched adjacent non-tumor tissues. CCK-8 assay and colony formation assay were used to demonstrate cell proliferation. Protein level was determined by western blotting. SPSS was used for statistical analyses.Results: MiR-1297 is repressed and PTEN activated in OSCC. Moreover, the miR-1297 overexpressing cell lines displayed a decrease in cell growth rate. And, suppression of miR-1297 reversed the cell growth rate. In addition, PTEN silencing display the similar pattern as miRNA-1297 overexpression to enhance OSCC cell growth.Conclusions MiRNA and gene expression changes are common event in OSCC. Our results suggest miR1297 may drive tumor progression through PTEN. And, miR-1297 could be a promising candidate for future investigation. (C) 2018 Production and hosting by Elsevier B.V. on behalf of King Saud University.