HLA Epitopes: The Targets of Monoclonal and Alloantibodies Defined.
HLA Epitopes: The Targets of Monoclonal and Alloantibodies Defined.
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DOI：
10.1155/2017/3406230
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发表时间：
2017
影响因子：
4.1
通讯作者：
Nguyen A
中科院分区：
文献类型：
--
作者：
El-Awar N;Jucaud V;Nguyen A
根据移植的体液理论，器官移植患者对人类白细胞抗原(人类白细胞抗原)的致敏会导致移植排斥反应。由于几乎所有移植受者的血清中都存在抗人类白细胞抗原抗体，致敏作用几乎无处不在。人类白细胞抗原的检测、分析和氨基酸序列测定以及计算机软件方面的进展有助于进一步了解抗体-抗原的反应性。通常认为，抗体与人类白细胞抗原结合。根据目前对表位的了解，更准确地描述抗体与其在HLA分子链表面的靶表位结合。表位存在于单个人类白细胞抗原上(私有表位)或由多个抗原共享(公共表位)。人类白细胞抗原检测中的交叉反应现象，通常被解释为抗原和抗体的交叉反应群(CREGs)，现在可以用公共表位清楚地解释。自2006年以来，我们定义并报道了194个人类白细胞抗原I类唯一表位，其中包括56个分离的人类白细胞抗原I类重链上的隐匿表位，83个人类白细胞抗原II类表位，60个人类白细胞抗原-DRB1表位，15个人类白细胞抗原DQB1表位，3个人类白细胞抗原DQA1表位，5个人类白细胞抗原DPB1表位，7个MICA表位。在这篇论文中，我们对我们的发现进行了总结。
Sensitization to human leukocyte antigens (HLA) in organ transplant patients causes graft rejection, according to the humoral theory of transplantation. Sensitization is almost ubiquitous as anti-HLA antibodies are found in almost all sera of transplant recipients. Advances in testing assays and amino acid sequencing of HLA along with computer software contributed further to the understanding of antibody-antigen reactivity. It is commonly understood that antibodies bind to HLA antigens. With current knowledge of epitopes, it is more accurate to describe that antibodies bind to their target epitopes on the surface of HLA molecular chains. Epitopes are present on a single HLA (private epitope) or shared by multiple antigens (public epitope). The phenomenon of cross-reactivity in HLA testing, often explained as cross-reactive groups (CREGs) of antigens with antibody, can be clearly explained now by public epitopes. Since 2006, we defined and reported 194 HLA class I unique epitopes, including 56 cryptic epitopes on dissociated HLA class I heavy chains, 83 HLA class II epitopes, 60 epitopes on HLA-DRB1, 15 epitopes on HLA-DQB1, 3 epitopes on HLA-DQA1, 5 epitopes on HLA-DPB1, and 7 MICA epitopes. In this paper, we provide a summary of our findings.