Hydrogel Glucose Sensor with In Vivo Stable Fluorescence Intensity Relying on Antioxidant Enzymes for Continuous Glucose Monitoring
Hydrogel Glucose Sensor with In Vivo Stable Fluorescence Intensity Relying on Antioxidant Enzymes for Continuous Glucose Monitoring
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DOI：
10.1016/j.isci.2020.101243
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发表时间：
2020-06
期刊：
影响因子：
5.8
通讯作者：
Jun Sawayama;T. Okitsu;Akihiro Nakamata;Y. Kawahara;S. Takeuchi
中科院分区：
文献类型：
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作者：
Jun Sawayama;T. Okitsu;Akihiro Nakamata;Y. Kawahara;S. Takeuchi
基于硼酸荧光强度的水凝胶葡萄糖传感器理论上有望通过促进持久的准确性来改善体内连续血糖监测（CGM）。然而，这些传感器通常在植入后降解，荧光强度随着时间的推移立即下降。在这里，我们描述了一种基于硼酸荧光强度的活稳定性水凝胶葡萄糖传感器，整合了两种抗氧化酶，超氧化物歧化酶（SOD）和过氧化氢酶。在体外保护芳基硼酸不被过氧化氢降解，并使大鼠体内基于硼酸的水凝胶葡萄糖传感器的荧光强度保持28天。这些抗氧化酶还允许水凝胶葡萄糖传感器连接到自制的半植入式CGM装置，以临床设置所需的精度跟踪大鼠的血糖浓度5小时。含有SOD和过氧化氢酶的基于硼酸的荧光强度水凝胶葡萄糖传感器可能构成一种新的vivoCGM策略。
Hydrogel glucose sensors with boronic acid-based fluorescence intensity theoretically hold promise to improvein vivocontinuous glucose monitoring (CGM) by facilitating long-lasting accuracy. However, these sensors generally degrade after implantation and the fluorescence intensity decreases immediately over time. Herein, we describe a hydrogel glucose sensor within vivostability based on boronic acid-based fluorescence intensity, integrating two antioxidant enzymes, superoxide dismutase (SOD), and catalase. These protected the arylboronic acid from being degraded by hydrogen peroxidein vitroand preserved the boronic acid-based fluorescence intensity of the hydrogel glucose sensors in rats for 28 days. These antioxidant enzymes also allowed the hydrogel glucose sensor attached to a homemade semi-implantable CGM device to trace blood glucose concentrations in rats for 5 h with the accuracy required for clinical settings. Hydrogel glucose sensors with boronic acid-based fluorescence intensity containing SOD and catalase could comprise a new strategy forin vivoCGM.