Identification of citrus tristeza virus strains by peptide maps of virion coat protein.
Identification of citrus tristeza virus strains by peptide maps of virion coat protein.
复制标题
通过病毒粒子外壳蛋白肽图鉴定柑橘 tristeza 病毒株。
DOI：
10.1094/phyto-80-692
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发表时间：
1990
期刊：
影响因子：
3.2
通讯作者：
R. Lee
中科院分区：
文献类型：
--
作者：
J. Guerri;P. Moreno;R. Lee
对6个柑橘衰退病毒(CTV)株系的病毒粒子外壳蛋白(CP)进行了半纯化的凝胶电泳法(SDS-PAGE)和凝胶特异蛋白条带的电洗脱。用五种内肽酶(木瓜酶、胰酶、V8酶、胰凝乳酶和热裂解酶)消化CPS和用SDS-PAGE分离得到的多肽图谱区分了6株CDT菌株(...
Virion coat proteins (CP) from six strains of citrus tristeza virus (CTV) were purified by gel electrphoresis (SDS-PAGE) of semipurified extracts and electroelution of the specific protein band from the gels. Peptide maps obtained by digestion of CPs with five endopeptidases (papain, trypsin, V8 protease, chymotrypsin, and thermolysin) and separation by SDS-PAGE distinguished the six CDT strains (...)