Meiotic differentiation during colony maturation in Saccharomyces cerevisiae.
Meiotic differentiation during colony maturation in Saccharomyces cerevisiae.
复制标题
酿酒酵母菌落成熟过程中的减数分裂分化。
DOI：
10.1007/s00294-002-0331-x
复制
发表时间：
2002
期刊：
影响因子：
2.5
通讯作者：
Honigberg,SaulM
中科院分区：
文献类型：
--
作者：
Purnapatre,Kedar;Honigberg,SaulM
当酵母菌落停止生长时，这些菌落边缘的细胞高效地从细胞分裂周期过渡到减数分裂。这种转变非常同步地发生，并且仅在菌落成熟的后期阶段发生。该转变发生在含有乙酸盐或低浓度葡萄糖的培养基上，但不会发生在含有高葡萄糖的培养基上。当IME1从质粒中过度表达时，高葡萄糖的抑制被克服。使用不同生长培养基的实验表明，集落中的减数分裂是由集落成熟时营养环境的变化引发的。在任何碳源中，孢子形成都需要HAP2，而葡萄糖抑制孢子形成需要GRR1。需要CLN3来抑制集落中的减数分裂，但在液体培养物中则不然，这表明介导集落中减数分裂转变的调节因子与在液体培养物中介导这种转变的调节因子不同。
As yeast colonies ceased growth, cells at the edge of these colonies transited from the cell division cycle into meiosis at high efficiency. This transition occurred remarkably synchronously and only at late stages of colony maturation. The transition occurred on medium containing acetate or low concentrations of glucose, but not on medium containing high glucose. The repression by high glucose was overcome whenIME1was overexpressed from a plasmid. Experiments with different growth media imply that meiosis in colonies is triggered by changes in the nutrient environment as colonies mature.HAP2is required to sporulate in any carbon source, whereasGRR1is required for glucose repression of sporulation.CLN3is required to repress meiosis in colonies but not in liquid cultures, indicating that the regulators that mediate the transition to meiosis in colonies are not identical to the regulators that mediate this transition in liquid cultures.