Selection of irrigation fluid to eradicate free cancer cells during head and neck cancer surgery
Selection of irrigation fluid to eradicate free cancer cells during head and neck cancer surgery
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DOI：
10.1002/hed.21773
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发表时间：
2012-04-01
影响因子：
2.9
通讯作者：
Sung, Myung-Whun
中科院分区：
文献类型：
--
作者：
Hah, J. Hun;Roh, Dong Hwan;Sung, Myung-Whun
游离癌细胞可以从暴露的肿瘤或破裂的肿瘤中溢出。我们在模拟头颈部手术的动物伤口模型中检测了各种冲洗液对游离癌细胞的杀细胞作用。用C3 H/HeJ小鼠和同系鳞状细胞癌（SCC VII）细胞制备癌细胞污染的伤口。使用蒸馏水、5%聚维酮碘、1.5%H2O2、生理盐水和顺铂冲洗5分钟。用不同浓度和暴露时间的聚维酮碘和蒸馏水进行体外肿瘤生长试验。在动物研究中，聚维酮碘显著抑制肿瘤生长。聚维酮碘引起体外肿瘤生长的实质性抑制，即使在0.05%的浓度下。暴露于1%聚维酮碘30秒后，癌细胞被完全抑制。聚维酮碘可优先选择用于头颈部手术期间的冲洗液，特别是当伤口疑似癌细胞污染时。(C)2011 Wiley Periodicals，Inc.头颈部34：546550，2012年
Background Free cancer cells can be spilled out from exposed tumors or ruptured tumors. We examined the cytocidal effect of various irrigation fluids on free cancer cells in an animal wound model mimicking head and neck surgery.Methods. Cancer cell-contaminated wounds were made with C3H/HeJ mice and syngeneic squamous cell carcinoma (SCC VII) cells. Distilled water, 5% povidone-iodine, 1.5% H2O2, normal saline, and cisplatin were used to irrigate for 5 minutes. In vitro tumor growth assays were done with different concentrations and exposure times of povidone-iodine and distilled water.Results. In the animal study, povidone-iodine significantly inhibited tumor growth. Povidone-iodine caused substantial inhibition of in vitro tumor growth, even at the concentration of 0.05%. After 30 seconds of exposure to 1% povidone-iodine, cancer cells were completely inhibited.Conclusions. Povidone-iodine could be selected preferentially for the irrigation fluid during head and neck surgery, especially when the wound is suspected of cancer cell contamination. (C) 2011 Wiley Periodicals, Inc. Head Neck 34: 546550, 2012