Visible reflectance hyperspectral imaging: characterization of a noninvasive, in vivo system for determining tissue perfusion.
Visible reflectance hyperspectral imaging: characterization of a noninvasive, in vivo system for determining tissue perfusion.
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DOI：
10.1021/ac011275f
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发表时间：
2002-04
影响因子：
7.4
通讯作者：
K. Zuzak;M. D. Schaeberle;E. Lewis;I. W. Levin
中科院分区：
文献类型：
--
作者：
K. Zuzak;M. D. Schaeberle;E. Lewis;I. W. Levin
我们描述了一种可见反射高光谱成像系统，用于在临床环境中对人体组织进行非侵入性、在体、定量分析。被摄体区域用石英-钨-卤素光源照明，反射光通过液晶可调谐滤光器(LCTF)进行光谱识别并成像到硅电荷耦合器件探测器上。LCTF在其有用的可见光谱范围(525-725 nm)内连续可调，平均半高带宽为0.38 nm，平均透过率为10.0%。在距系统5.5英尺的地方放置标准分辨率目标，可获得直径17厘米、最佳空间分辨率为0.45毫米的视场。测量的反射光谱根据表观吸光度进行量化，并格式化为高光谱图像立方体。作为一个临床例子，我们研究了一个血管功能障碍的模型，该模型涉及组织再灌注期间的缺血和反应性充血。在该模型中，基于525-645 nm区域的氧合血红蛋白和脱氧血红蛋白信号的光谱图像使用多元最小二乘回归分析进行去卷积，以可视化特定皮肤组织区域中氧合血红蛋白和脱氧血红蛋白的百分比的空间分布。
We characterize a visible reflectance hyperspectral imaging system for noninvasive, in vivo, quantitative analysis of human tissue in a clinical environment. The subject area is illuminated with a quartz-tungsten-halogen light source, and the reflected light is spectrally discriminated by a liquid crystal tunable filter (LCTF) and imaged onto a silicon charge-coupled device detector. The LCTF is continuously tunable within its useful visible spectral range (525-725 nm) with an average spectral full width at half-height bandwidth of 0.38 nm and an average transmittance of 10.0%. A standard resolution target placed 5.5 ft from the system results in a field of view with a 17-cm diameter and an optimal spatial resolution of 0.45 mm. The measured reflectance spectra are quantified in terms of apparent absorbance and formatted as a hyperspectral image cube. As a clinical example, we examine a model of vascular dysfunction involving both ischemia and reactive hyperemia during tissue reperfusion. In this model, spectral images, based upon oxyhemoglobin and deoxyhemoblobin signals in the 525-645-nm region, are deconvoluted using a multivariate least-squares regression analysis to visualize the spatial distribution of the percentages of oxyhemoglobin and deoxyhemoglobin in specific skin tissue areas.