PHENOTYPICAL CHARACTERIZATION OF NEURONS EXPRESSING THE DOPAMINE D-3 RECEPTOR IN THE RAT-BRAIN
PHENOTYPICAL CHARACTERIZATION OF NEURONS EXPRESSING THE DOPAMINE D-3 RECEPTOR IN THE RAT-BRAIN
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DOI：
10.1016/0306-4522(94)00527-c
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发表时间：
1995-04-01
期刊：
影响因子：
3.3
通讯作者：
SOKOLOFF, P
中科院分区：
文献类型：
--
作者：
DIAZ, J;LEVESQUE, D;SOKOLOFF, P
我们已经建立了多巴胺D-3受体的细胞分布，使用氚标记的7-羟基-N-N-二-正丙基-2-氨基四氢化萘和互补的RNA探针，以可视化放射自显影结合研究中的蛋白质和原位杂交的基因转录本，分别。使用这两种标记物的研究证实了D-3受体在少数大脑区域的有限表达，即主要是腹侧纹状体复合体、黑质腹侧被盖区和小脑。在延髓核，D-3受体主要表达在中型神经元的吻极和腹内侧壳分区，但不是核心或隔极，即表达D-2受体的延髓分区。在腹内侧壳，约60%的D-3受体表达神经元的神经降压素神经元，推测投射到腹侧苍白球。在Calleja岛，D-3受体结合和信使RNA在整个颗粒细胞群中都很丰富。已知这些细胞与多巴胺能轴突进行稀疏接触，并且还表达D-1受体。中脑黑质外侧部和腹侧被盖区的少数多巴胺神经元中检测到低水平的D-3信使RNA。此外，在内侧黑质和外侧腹侧被盖区检测到一些D-3受体结合，而不是信使RNA，那里的受体可能位于突触前的传入。在原小脑中，第9和第10小叶的浦肯野细胞胞体表达D-3受体信使RNA，而结合位点位于分子层，其中发现了相应的树突，但没有已知的来自中脑的多巴胺能投射。在一些接受低多巴胺神经支配的大脑区域中D-3受体基因表达的出现支持了该受体可能介导铁的假设多巴胺的突触作用
We have established the cellular distribution of the dopamine D-3 receptor using tritiated 7-hydroxy-N-N-di-n-propyl-2-aminotetralin and a complementary RNA probe to visualize autoradiographically the protein in binding studies and the gene transcripts by in situ hybridization, respectively. Studies with these two markers confirm the restricted expression of the D-3 receptor in few brain areas, i.e. mainly the ventral striatal complex, the substantia nigra-ventral tegmental area and the cerebellum. In nucleus accumbens, the D-3 receptor was mainly expressed in medium-sized neurons of the rostral pole and ventromedial shell subdivisions, but not of the core or septal pole, i.e. accumbal subdivisions expressing the D-2 receptor. In the ventromedial shell, about 60% of the D-3 receptor-expressing neurons were neurotensin neurons, presumably projecting to the ventral pallidum. In the islands of Calleja, both D-3 receptor binding and messenger RNA were abundant in the entire population of granule cells. These cells are known to make sparse contacts with dopaminergic axons and also to express the D-1 receptor. In the mesencephalon, low levels of D-3 messenger RNA were detected in few dopamine neurons of substantia nigra pars lateralis and ventral tegmental area. In addition, some D-3 receptor binding but not messenger RNA was detected in medial substantia nigra and lateral ventral tegmental area, where the receptor is presumably located presynaptically on afferents. In the archicerebellum, Purkinje cell perikarya in lobules 9 and 10 expressed the D-3 receptor messenger RNA, whereas binding sites were found in the molecular layer, where corresponding dendrites but no known dopaminergic projection from mesencephalon are found.The occurrence of D-3 receptor gene expression in some brain areas receiving low dopamine innervation supports the hypothesis that this receptor may mediate Iron-synaptic actions of dopamine.