NUCLEAR ASSEMBLY IS INDEPENDENT OF LINKER HISTONES
NUCLEAR ASSEMBLY IS INDEPENDENT OF LINKER HISTONES
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DOI：
10.1073/pnas.91.26.12477
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发表时间：
1994-12-20
影响因子：
11.1
通讯作者：
WOLFFE, AP
中科院分区：
文献类型：
--
作者：
DASSO, M;DIMITROV, S;WOLFFE, AP
使用爪蟾卵的无细胞提取物，将浓缩的精子染色质转化为DNA复制能力原核，研究了连接蛋白在功能性细胞核组装中的作用。当从提取物中去除连接组蛋白时，所得到的原核与完整提取物中形成的原核无法区分。功能核膜、核层和预复制中心的组装允许相同的DNA复制效率。因此，连接组蛋白对于能够进行DNA复制的形态正常的细胞核的组装是不需要的。
The role of linker histones in the assembly of functional nuclei was examined with the use of a cell-free extract of Xenopus eggs that transforms condensed sperm chromatin into DNA replication-competent pronuclei. When linker histones were removed from the extract, the resultant pronuclei were indistinguishable from those formed in the complete extract. The assembly of functional nuclear membrane, nuclear lamina, and prereplication centers allowed identical DNA replication efficiencies. Thus, linker histones are not required for the assembly of morphologically normal nuclei capable of DNA replication.