Multiplex Lateral-Flow Test Strips Fabricated by Two-Dimensional Shaping
Multiplex Lateral-Flow Test Strips Fabricated by Two-Dimensional Shaping
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DOI：
10.1021/am800043z
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发表时间：
2009-01-01
影响因子：
9.5
通讯作者：
Sibbett, Scott S.
中科院分区：
文献类型：
--
作者：
Fenton, Erin M.;Mascarenas, Monica R.;Sibbett, Scott S.
我们已经制造了基于纸和硝化纤维素的横向流动装置，这些装置通过计算机控制的刀在二维空间中成形。将所得的星星、枝状大烛台和其他结构用多种生物测定试剂点样以产生多重侧向流测定。我们还制作了层状复合材料，其中多孔硝化纤维素介质夹在乙烯基和聚酯塑料薄膜。这最大限度地减少了蒸发，保护检测表面免受污染和脱水，并消除了对通常用于包装商业侧流诊断条的传统硬塑料盒架的需要。所报道的制造方法是新颖的、低成本的，并且非常适合于（i）在资源贫乏地区的制造和采用，（ii）原型开发，（iii）大批量制造，以及（iii）改善操作员错误率。
We have fabricated paper- and nitrocellulose-based lateral-flow devices that are shaped in two dimensions by a computer-controlled knife. The resulting star, candelabra, and other structures are spotted with multiple bioassay reagents to produce multiplex lateral-flow assays. We have also fabricated laminar composites in which porous nitrocellulose media are sandwiched between vinyl and polyester plastic films. This minimizes evaporation, protects assay surfaces from contamination and dehydration, and eliminates the need for the conventional hard plastic cassette holders that are typically used to package commercial lateral-Flow diagnostic strips. The reported fabrication method is novel, low-cost, and well-suited to (i) fabrication and adoption in resource-poor areas, (ii) prototype development, (iii) high-volume manufacturing, and (iii) improving rates of operator error.