Changes in the transcriptional fingerprint of satellite glial cells following peripheral nerve injury
Changes in the transcriptional fingerprint of satellite glial cells following peripheral nerve injury
复制标题
周围神经损伤后卫星胶质细胞转录指纹图谱的变化
DOI：
10.1002/glia.23785
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发表时间：
2020-02-11
期刊：
影响因子：
6.2
通讯作者：
Vaegter, Christian B.
中科院分区：
文献类型：
--
作者：
Jager, Sara E.;Pallesen, Lone T.;Vaegter, Christian B.
卫星胶质细胞(SGCs)是包被周围感觉神经元和自主神经元胞体的动态平衡细胞。各种各样的神经元应激源触发SGCs的激活，例如，通过调节神经元活动而导致神经病理性疼痛。然而，与神经系统的神经元和其他神经胶质细胞相比，SGCs受到了适度的科学关注，对SGC生物学知之甚少，这可能是由于在体内和体外研究它们所面临的实验挑战。利用最近发展的一种从背根节(DRG)中提取SGC RNA的方法，我们采用了一种系统的方法，通过下一代测序来表征SGC的转录指纹图谱，并首次获得了SGC损伤反应的概述。我们的RNA测序数据可以在Excel格式的支持信息中轻松访问。他们揭示了SGCs富含与免疫系统和细胞间通讯相关的基因。对神经损伤范例中SGC转录变化的分析表明，损伤后3天和14天的反应不同，表明SGC功能随时间的动态调节。在两个时间点都观察到了与胆固醇合成相关的几个基因的显著下调。相比之下，与免疫系统相关的基因簇(MHC蛋白复合体和白细胞迁移)的调节主要在14天后观察到。最后，我们证明，在神经损伤后，巨噬细胞在物理上更接近小的和大的背根节神经元，以前报道的损伤诱导的SGCs增殖实际上可能是增殖的巨噬细胞。
Satellite glial cells (SGCs) are homeostatic cells enveloping the somata of peripheral sensory and autonomic neurons. A wide variety of neuronal stressors trigger activation of SGCs, contributing to, for example, neuropathic pain through modulation of neuronal activity. However, compared to neurons and other glial cells of the nervous system, SGCs have received modest scientific attention and very little is known about SGC biology, possibly due to the experimental challenges associated with studying them in vivo and in vitro. Utilizing a recently developed method to obtain SGC RNA from dorsal root ganglia (DRG), we took a systematic approach to characterize the SGC transcriptional fingerprint by using next-generation sequencing and, for the first time, obtain an overview of the SGC injury response. Our RNA sequencing data are easily accessible in supporting information in Excel format. They reveal that SGCs are enriched in genes related to the immune system and cell-to-cell communication. Analysis of SGC transcriptional changes in a nerve injury-paradigm reveal a differential response at 3 days versus 14 days postinjury, suggesting dynamic modulation of SGC function over time. Significant downregulation of several genes linked to cholesterol synthesis was observed at both time points. In contrast, regulation of gene clusters linked to the immune system (MHC protein complex and leukocyte migration) was mainly observed after 14 days. Finally, we demonstrate that, after nerve injury, macrophages are in closer physical proximity to both small and large DRG neurons, and that previously reported injury-induced proliferation of SGCs may, in fact, be proliferating macrophages.