THE FATE OF HUMAN GLIAL-CELLS FOLLOWING TRANSPLANTATION IN NORMAL RODENTS AND RODENT MODELS OF NEURODEGENERATIVE DISEASE
THE FATE OF HUMAN GLIAL-CELLS FOLLOWING TRANSPLANTATION IN NORMAL RODENTS AND RODENT MODELS OF NEURODEGENERATIVE DISEASE
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DOI：
10.1016/0006-8993(95)00753-d
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发表时间：
1995-10-09
期刊：
影响因子：
2.9
通讯作者：
LOW, WC
中科院分区：
文献类型：
--
作者：
PUNDT, LL;KONDOH, T;LOW, WC
对脑中异种移植的研究以前集中在移植神经元的解剖和功能整合上。最近，星形胶质细胞被认为在移植后具有复杂的功能，并且正在研究以确定它们在移植中的作用。本研究旨在探讨人星形胶质细胞移植到啮齿类动物纹状体后的迁移。本研究使用了通过选择性流产和自然流产获得的人供体胎儿（妊娠9-16周）。移植后，供体星形胶质细胞用抗人胶质细胞酸性蛋白的抗血清标记。我们的研究结果表明，星形胶质细胞元素从所有三种组织类型都能够纳入宿主脑，并有一种趋势，遵循白色物质束（如胼胝体，内囊，和纤维束在纹状体）。源自纹状体和丘脑的人星形胶质细胞表现出广泛的迁移，而腹侧中脑移植动物的迁移更为有限。中脑移植的动物在移植物内表现出阳性星形胶质细胞，突起（很少的细胞体）延伸到邻近宿主纹状体的白色物质中。在所有移植类型中均观察到表现出不成熟形态的星形胶质细胞，但在纹状体移植动物中最常见。本研究中观察到的星形胶质细胞迁移的程度和形态反映了发育中人脑的区域差异。这些结果证实并扩展了先前关于脑内移植后胶质细胞迁移的研究。
Investigations on xenografting in the brain have previously focused on the anatomical and functional integration of the transplanted neurons. More recently, astrocytes are being implicated as having complex functions following transplantation, and are being investigated to determine their role(s) in transplantation. The present study was undertaken to investigate the migration of human astrocytes following transplantation of thalamic, striatal, and mesencephalic tissue into the rodent striatum. Human donor fetuses (9-16 weeks in gestation) obtained through elective and spontaneous abortions were utilized in this study. Following transplantation, donor astrocytes were labeled with an antiserum directed against human glial fibrillary acidic protein. Our results demonstrate that astrocytic elements from all three tissue types are capable of incorporating into the host brain, and have a tendency to follow white matter tracts (such as the corpus callosum, internal capsule, and fiber bundles in the striatum). Human astrocytes originating from the striatum and thalamus exhibited extensive migration, while migration was more limited in animals with ventral mesencephalon transplants. Ventral mesencephalon transplanted animals demonstrated positive astrocytes within the transplant, with processes (very few cell bodies) extending into white matter of adjacent host striatum. Astrocytes demonstrating immature morphology were observed with all transplant types, but were most prevalent in the striatal transplanted animals. The extent of astrocyte migration and the morphologies observed in this study reflect regional differences of the developing human brain. These results confirm and extend previous investigations on glial cell migration following transplantation in the brain.