Cultivar Identification of Evergreen Azalea with RAPD Method
Cultivar Identification of Evergreen Azalea with RAPD Method
复制标题
RAPD法鉴定常绿杜鹃花品种
DOI：
10.2503/jjshs.64.611
复制
发表时间：
1995
影响因子：
--
通讯作者：
K. Takayanagi
中科院分区：
文献类型：
--
作者：
N. Kobayashi;R. Takeuchi;T. Handa;K. Takayanagi
利用随机扩增多态性DNA技术对杜鹃常绿品种进行了鉴定，确定了杜鹃DNA的最佳提取方法。被调查过了。在几乎所有原产于日本的杜鹃属植物和一些品种群中，无论是SDS法还是CTAB法都不足以从叶片中提取清洁的DNA。但是，在Greenwood等人之后使用了改进的CTAB法。(1989)，我们从本实验所使用的材料中获得了高质量的DNA。利用上述最佳提取方法并结合随机扩增多态性DNA分析，可以区分不同品种杜鹃的差异。在测试的100个引物中，有16个产生了明显的、明确的多态DNA条带。利用引物OPK-19或OPK-20对江户麒麟组(9个品种)和库鲁姆杜鹃组(14个品种)的品种进行了清晰的区分。在Satsuki组中，这两个引物可用于区分‘口赞’和它的芽生品种。本研究的结果有力地表明了随机扩增多态性DNA技术在常绿杜鹃花品种鉴定中的应用价值。
To use RAPD method for the identification of evergreen azalea cultivars, the optimum method for DNA extraction in Rhododendron spp. was investigated. Of almost all Rhododendron species native to Japan and some cultivar groups, neither SDS nor CTAB method was sufficient to isolate clean DNA from leaves. But, by using the modified CTAB method after Greenwood et al. (1989), we obtained a good yield of high quality DNA from the materials used in this experiment. Using the optimum extraction method mentioned above and RAPD analysis, differences among azalea cultivars could be distinguished. Sixteen out of 100 primers tested produced obvious, definitive polymorphic DNA bands. Cultivars in the Edo-Kirishima group (9 cultivars) or Kurume azalea group (14 cultivars) were clearly recognized from one another by using primer OPK-19 or OPK-20. In the Satsuki group, these two primers could be used to discern 'Kouzan' from its bud sport cultivars. The results of this study strongly suggest that RAPD method is a very useful tool for cultivar identification in evergreen azalea.
Honda, H.：“一种使用非放射性 rDNA 作为探针来鉴定杂交体的简单而有效的方法。”
DOI：
--
发表时间：
--
期刊：
影响因子：
--
作者：
通讯作者：
--