Opacity factor from group A streptococci is an apoproteinase.
Opacity factor from group A streptococci is an apoproteinase.
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A 组链球菌的不透明因子是一种脱辅基蛋白酶。
DOI：
10.1111/j.1574-6968.1990.tb04118.x
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发表时间：
1990
影响因子：
2.1
通讯作者：
D. Martin
中科院分区：
文献类型：
--
作者：
G. Saravani;D. Martin
不透明因子（OF）是一种酶，由A组链球菌的某些血清型加工而成，在哺乳动物血清中产生乳光。OF已被指定为脂蛋白酶。脂蛋白是复杂的结构，许多酶参与其催化。因此，我们着手确定许多酶中的哪一种可能是。结果表明，OF使血清中高密度脂蛋白（HDL）不溶，并改变其电泳迁移率。电子显微镜观察显示OF引起HDL聚集和分子形状改变。OF特异性地将HDL的载脂蛋白AI切割成两个片段，SDS-PAGE显示。因此，我们将OF命名为脱蛋白酶。
Opacity factor (OF) is an enzyme, elaborated by certain serotypes of group A streptococci, which produces opalescence in mammalian sera. OF has been designated a lipoproteinase. Lipoproteins are complex structures and many enzymes are involved in their catalysis. We therefore set out to establish which of the many enzymes OF could be. Results showed that OF rendered high density lipoprotein (HDL) insoluble, accounting for the opalescence in serum, and altered its electrophoretic mobility. Electron microscopy revealed that OF caused an aggregation of HDL and an alteration in molecule shape. OF specifically split apoprotein AI of HDL into two fragments demonstrable by SDS-PAGE. We therefore designate OF as an apoproteinase.